Nuclear envelope and nuclear pore complex structure and organization in tobacco BY-2 cells
Nuclear envelope and nuclear pore complex structure and organization in tobacco BY-2 cells
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DOI：
10.1111/j.1365-313x.2009.03865.x
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发表时间：
2009-07-01
期刊：
影响因子：
7.2
通讯作者：
Goldberg, Martin W.
中科院分区：
文献类型：
--
作者：
Fiserova, Jindriska;Kiseleva, Elena;Goldberg, Martin W.
核包膜（NE）是真核细胞的基本结构，具有双重作用：它将两个不同的区室分开，并通过核孔复合物（NPCs）使它们之间能够进行通信。对植物中NPCs和NE的结构组织知之甚少。我们从烟草BY-2细胞的NE两侧研究了NPCs的结构。我们发现分裂核和静止核的NPCs在结构上存在差异。重要的是，我们还追踪了NPC的组织模式，并观察了NPC在核表面上的非随机分布。最后，我们观察到一种有组织的丝状蛋白结构，它位于核膜之下，并将npc相互连接起来。这些结果在当前对高等真核生物中NE结构和功能的理解的背景下进行了讨论。
P>The nuclear envelope (NE) is a fundamental structure of eukaryotic cells with a dual role: it separates two distinct compartments, and enables communication between them via nuclear pore complexes (NPCs). Little is known about NPCs and NE structural organization in plants. We investigated the structure of NPCs from both sides of the NE in tobacco BY-2 cells. We detected structural differences between the NPCs of dividing and quiescent nuclei. Importantly, we also traced the organizational pattern of the NPCs, and observed non-random NPC distribution over the nuclear surface. Lastly, we observed an organized filamentous protein structure that underlies the inner nuclear membrane, and interconnects NPCs. The results are discussed within the context of the current understanding of NE structure and function in higher eukaryotes.