Horizontal Intracorneal Swirling Water Migration Indicative of Corneal Endothelial Function
Horizontal Intracorneal Swirling Water Migration Indicative of Corneal Endothelial Function
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DOI：
10.1167/iovs.14-14762
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发表时间：
2014-12-01
影响因子：
4.4
通讯作者：
Ohashi, Yuichi
中科院分区：
文献类型：
--
作者：
Inoue, Tomoyuki;Kobayashi, Takeshi;Ohashi, Yuichi
目的.目的：验证角膜水平面内是否存在水分迁移的假说，并探讨其发生机制。将荧光素溶液注射到兔的正常和水肿角膜中，观察并记录荧光素溶液随时间的移动。在正常角膜中，水流的特征在于在基质中从中心到周边的涡旋运动。荧光素溶液最终扩散并占据整个角膜，表明水的水平角膜内漩涡。相比之下，当角膜内皮被前房内注射防腐剂损伤以产生角膜水肿时，没有发生水迁移，这表明角膜内皮功能的完整性对于水迁移至关重要。注射钠-钾泵抑制剂后，水迁移停止，表明该酶是角膜中生理性水迁移所必需的。随着角膜内皮功能的恢复，水开始迁移，并且局灶性水肿保持在周边，在该水肿区域没有水迁移。我们报告的第一次存在的水平水迁移在角膜中的漩涡模式（即。例如，角膜内皮细胞中的泵功能产生的水的角膜内旋转迁移），这可以补充在垂直平面中角膜水肿发展的传统概念。该动态水循环系统可以参与提高整个角膜中的水转移效率。
PURPOSE. To test our hypothesis about whether there is water migration in the horizontal corneal plane and investigate its developmental mechanism.METHODS. A fluorescein solution was intrastromally injected into normal and edematous corneas of rabbits, and the movement of the fluorescein solution was observed and recorded over time.RESULTS. In normal corneas, the water flow was characterized by a swirling movement from the center to the periphery in the stroma. The fluorescein solution ultimately spread and occupied the entire cornea, indicating horizontal intracorneal swirling of water. In contrast, when the corneal endothelia were injured by intracameral injection of a preservative to create corneal edema, no water migration occurred, suggesting that the integrity of the corneal endothelial function is essential for water migration. The water migration stopped with injection of a sodium-potassium pump inhibitor, indicating that the enzyme is necessary for physiologic water migration in the cornea. With recovery of corneal endothelial function, the water migration began, and focal edema remained in the periphery with no water migration in this edematous area.CONCLUSIONS. We report for the first time the presence of horizontal water migration in the cornea in a swirling pattern (i. e., intracorneal swirling migration of water, generated by the pump function in the corneal endothelial cells), which may supplement the conventional concept of development of corneal edema in the vertical plane. This dynamic water circulatory system may be involved in increasing the efficiency of the water transfer in the entire cornea.