Long-term interleukin 2-dependent growth and cytotoxic activity of tumor-infiltrating lymphocytes from human squamous cell carcinomas of the head and neck.
Long-term interleukin 2-dependent growth and cytotoxic activity of tumor-infiltrating lymphocytes from human squamous cell carcinomas of the head and neck.
复制标题
人头颈鳞状细胞癌肿瘤浸润淋巴细胞的长期白细胞介素 2 依赖性生长和细胞毒活性。
作者：
D. S. Heo;T. Whiteside;Jonas T. Johnson;K. Chen;E. Barnes;R. Herberman
本文对16例头颈部鳞状细胞癌（SCCH&N）和4例非鳞状细胞癌的肿瘤浸润淋巴细胞（TIL）进行了研究。免疫过氧化物酶原位染色发现肿瘤主要为CD_2 ~+ CD_3 ~+细胞浸润，30-50%的T淋巴细胞为HLA-DR和转铁蛋白受体阳性。它们还含有稀少的NKH 1+细胞。当TIL和自体外周血淋巴细胞（A-PBL）在1,000 U/ml的重组白细胞介素2（rIL 2）中培养时，除3例外，所有TIL均增殖，除2例外，所有A-PBL均增殖。TIL的扩增经常好于A-PBL，但并非总是如此。在维持长达88天的长期培养物中，TIL的中位扩增为100倍，A-PBL的中位扩增为31倍。从未经处理的原发性SCCH&N获得的TIL最初延迟了对rIL 2的增殖反应长达20天，但随后生长良好。相比之下，来自转移性SCCH&N的TIL和A-PBL要么不增殖，要么增殖反应延迟长达40或50天。A-PBL在早期（培养第10-20天）测试时显示出对培养的和新鲜的肿瘤细胞靶的最高细胞毒性活性，而TIL在培养后期（第20-30天）最活跃。在单个培养物的基础上，TIL比A-PBL具有更高的抗肿瘤细胞毒性。到第80天，大多数TIL培养物的裂解活性下降到检测不到的水平。在大多数TIL培养物中，CD 3 + Leu 19-T淋巴细胞是主要的扩增细胞群。然而，如细胞分选实验所示，这些细胞在TIL或A-PBL培养物中是抗肿瘤细胞毒性的不良介体。抗肿瘤效应细胞表达CD 3-Leu 19+和/或CD 3 + Leu 19+表型。在Giemsa染色涂片上，这两种类型的IL 2扩增的效应细胞具有大颗粒淋巴细胞的形态。我们的研究结果表明，从人SCCH&N的TIL可以扩大，并达到高水平的抗肿瘤效应功能，在长期培养与rIL 2。
Tumor-infiltrating lymphocytes (TIL) from 16 squamous cell carcinomas of head and neck (SCCH&N) and four nonsquamous cell carcinomas were studied. By immunoperoxidase staining in situ, the tumors studied were found to be infiltrated mainly by CD2+CD3+ cells, and 30-50% of the T-lymphocytes were HLA-DR positive and transferrin-receptor positive. They also contained scarce NKH1+ cells. When TIL as well as autologous peripheral blood lymphocytes (A-PBL) were cultured in 1,000 U/ml of recombinant interleukin 2 (rIL2), TIL proliferated in all but three cases, and A-PBL proliferated in all but two cases. Frequently, but not always, TIL expanded better than A-PBL. The median expansion for TIL was 100-fold and that for A-PBL was 31-fold in long-term cultures maintained for up to 88 days. TIL obtained from untreated primary SCCH&N were initially delayed for up to 20 days in their proliferative response to rIL2, but then grew well. In contrast, TIL and A-PBL from metastatic SCCH&N either did not proliferate or were delayed in their proliferative response for up to 40 or 50 days. A-PBL, when tested early (days 10-20 in culture), showed the highest cytotoxic activity against cultured and fresh tumor-cell targets, whereas TIL were most active later in culture (days 20-30). On a per culture basis, TIL achieved higher antitumor cytotoxicity than A-PBL. By day 80, lytic activities of most TIL cultures declined to undetectable levels. CD3+Leu19- T-lymphocytes were the major expanding cell population in most TIL cultures. However, these cells were poor mediators of antitumor cytotoxicity in TIL or A-PBL cultures as shown in cell sorting experiments. The antitumor effector cells expressed CD3-Leu19+ and/or CD3+Leu19+ phenotypes. On Giemsa-stained smears, these two types of IL2-expanded effector cells had the morphology of large granular lymphocytes. Our results indicate that TIL from human SCCH&N could be expanded and reach high levels of antitumor effector function in long-term cultures with rIL2.
人乳腺癌中主要组织相容性抗原和炎症细胞浸润的免疫组织学特征。
DOI：
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发表时间：
1983
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Bhan,AK;DesMarais,CL
通讯作者：
DesMarais,CL
浸润人肺部肿瘤的淋巴细胞中自然杀伤细胞毒活性的抑制。
影响因子：
11.2
作者：
Moy,PM;Holmes,EC;Golub,SH
通讯作者：
Golub,SH