STRUCTURE OF FULLY HYDRATED BILAYER DISPERSIONS
STRUCTURE OF FULLY HYDRATED BILAYER DISPERSIONS
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DOI：
10.1016/0005-2736(88)90268-4
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发表时间：
1988-07-07
期刊：
影响因子：
--
通讯作者：
WIENER, MC
中科院分区：
文献类型：
--
作者：
NAGLE, JF;WIENER, MC
一个系统的形式主义的发展，显示了如何的结果，绝对特定体积的多层脂质分散体可以结合衍射研究的结果，以获得定量表征的平均结构的完全水合的脂质双层。所获得的参数是每分子的面积、双层、水层、烃链层和头基层的厚度和体积，以及在适当的情况下，烃链的倾斜角。在DPPC的C阶段的情况下，这种形式主义导致检测到三个数据之间的不一致。DPPC和DLPE的G阶段的结果是在合理的协议，虽然更全面的比，以前的工作，使用较少的数据和方程。DPPC的F相的各种衍射数据是不一致的，它示出了这种不一致影响双层结构的结果。McIntosh和Simon最近利用凝胶相结构获得流体相结构的方法被稍微修改以获得DLPE的F相的结果。获得的平均亚甲基和甲基的体积在流体相的方法进行了严格的检查。
A systematic formalism is developed that shows how the results for absolute specific volumes of multilamellar lipid dispersions may be combined with results from diffraction studies to obtain quantitative characterizations of the average structure of fully hydrated lipid bilayers. Quantities obtained are the area per molecule, the thickness and volumes of the bilayer, the water layer, the hydrocarbon chain layer and the headgroup layer, and where appropriate, the tilt angle of the hydrocarbon chains. In the case of the C phase of DPPC this formalism leads to the detection of inconsistencies between three data. Results for the G phases of DPPC and DLPE are in reasonable agreement with, though more comprehensive than, previous work that used fewer data and equations. Various diffraction data for the F phase of DPPC are in disagreement and it is shown this disagreement affects results for the bilayer structure. A recent method of McIntosh and Simon for obtaining fluid phase structure utilizing gel phase structure is slightly modified to obtain results for the F phase of DLPE. Methods of obtaining the average methylene and methyl volumes in the fluid phases are critically examined.