DEVELOPMENT OF COWPEA MOSAIC-VIRUS AS A HIGH-YIELDING SYSTEM FOR THE PRESENTATION OF FOREIGN PEPTIDES
DEVELOPMENT OF COWPEA MOSAIC-VIRUS AS A HIGH-YIELDING SYSTEM FOR THE PRESENTATION OF FOREIGN PEPTIDES
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DOI：
10.1006/viro.1994.1417
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发表时间：
1994-08-01
期刊：
影响因子：
3.7
通讯作者：
LOMONOSSOFF, GP
中科院分区：
文献类型：
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作者：
PORTA, C;SPALL, VE;LOMONOSSOFF, GP
最近已经表明，豇豆植物可以被含有来自口蹄疫病毒的抗原位点的豇豆花叶病毒（CPMV）嵌合体感染（Usha等人，Virology 197，366-374，1993）。对这种感染过程中产生的子代RNA的分析表明，插入的序列在连续传代过程中迅速丢失，可能是通过同源重组的过程。利用从这种分析中获得的信息，我们重新设计了嵌合体，使它们现在在遗传上稳定。修饰的构建体已用于获得大量纯化的病毒颗粒，其表达源自人鼻病毒14（HRV-14）和人免疫缺陷病毒1型（HIV-1）的表位。嵌合病毒颗粒具有插入序列的抗原特性，并且在HRV-14衍生构建体的情况下，已经显示插入的表位在兔中具有免疫原性。这些结果表明，CPMV可以用作用于呈递外源肽序列的高产系统。(C)1994年出版社出版。
It has recently been shown that cowpea plants can be infected with a cowpea mosaic virus (CPMV) chimera containing an antigenic site from foot-and-mouth disease virus (Usha et al., Virology 197, 366-374, 1993). Analysis of progeny RNA produced during such an infection has revealed that the inserted sequence is rapidly lost during serial passaging, probably by a process of homologous recombination. Using the information gained from this analysis, we have redesigned the chimeras in such a way that they are now genetically stable. The modified constructs have been used to obtain large quantities of purified virus particles expressing epitopes derived from human rhinovirus 14 (HRV-14) and human immunodeficiency virus type 1 (HIV-1). The chimeric virus particles possess the antigenic properties of the inserted sequence and, in the case of the HRV-14-derived construct, it has been shown that the inserted epitope is immunogenic in rabbits. These results demonstrate that CPMV can be used as a high-yielding system for the presentation of foreign peptide sequences. (C) 1994 Academic Press, Inc.