Effect of ciprofloxacin on killing of Actinobacillus actinomycetemcomitans by polymorphonuclear leukocytes.
Effect of ciprofloxacin on killing of Actinobacillus actinomycetemcomitans by polymorphonuclear leukocytes.
复制标题
环丙沙星对多形核白细胞杀灭伴放线放线杆菌的影响。
DOI：
10.1128/aac.46.6.1980-1984.2002
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发表时间：
2002
影响因子：
4.9
通讯作者：
Walters,JohnD
中科院分区：
文献类型：
--
作者：
Cacchillo,DavidA;Walters,JohnD
Actinobacillus actinomycetemcomitans, a pathogen associated with aggressive periodontitis, resists phagocytic killing by polymorphonuclear leukocytes (PMNs). It is susceptible to ciprofloxacin, which PMNs actively accumulate. This study tested the hypothesis that ciprofloxacin-loaded PMNs are more effective at killingA. actinomycetemcomitansthan control PMNs. Isolated human PMNs were loaded by brief incubation with 0.5 μg of ciprofloxacin/ml. Opsonized bacteria (ATCC 43718) were incubated at 37°C with control and ciprofloxacin-loaded PMNs and in the presence and absence of 0.5 μg of ciprofloxacin/ml. When assayed at bacteria-to-PMN ratios of 30:1 and 90:1, ciprofloxacin-loaded PMNs killed significantly more bacteria and achieved significantly shorter half times for killing than control PMNs (P< 0.05; Tukey's test). At ratios of 3:1 and 10:1, these differences were not significant.
