ISOLATION OF AN ADDITIONAL MEMBER OF THE FIBROBLAST GROWTH-FACTOR RECEPTOR FAMILY, FGFR-3
ISOLATION OF AN ADDITIONAL MEMBER OF THE FIBROBLAST GROWTH-FACTOR RECEPTOR FAMILY, FGFR-3
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DOI：
10.1073/pnas.88.4.1095
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发表时间：
1991-02-01
影响因子：
11.1
通讯作者：
HAYMAN, MJ
中科院分区：
文献类型：
--
作者：
KEEGAN, K;JOHNSON, DE;HAYMAN, MJ
成纤维细胞生长因子是参与多种活性（包括有丝分裂发生、血管生成和伤口愈合）的多肽生长因子家族。 成纤维细胞生长因子受体（FGFR）先前已在鸡、小鼠和人中鉴定，并且已显示含有具有两个或三个免疫球蛋白样结构域的细胞外结构域、跨膜结构域和胞质酪氨酸激酶结构域。 我们已经分离出另一种酪氨酸激酶受体的人cDNA，该受体与先前描述的FGFR高度同源。 该受体cDNA在COS细胞中的表达指导125-kDa糖蛋白的表达。 我们证明，这种cDNA编码的生物活性受体显示，人类酸性和碱性成纤维细胞生长因子激活这种受体的Ca-45（2+）流出测定。 这些数据证实了FGFR家族中另外一个成员的存在，我们将其命名为FGFR-3。
The fibroblast growth factors are a family of polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing. Fibroblast growth factor receptors (FGFRs) have previously been identified in chicken, mouse, and human and have been shown to contain an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. We have isolated a human cDNA for another tyrosine kinase receptor that is highly homologous to the previously described FGFR. Expression of this receptor cDNA in COS cells directs the expression of a 125-kDa glycoprotein. We demonstrate that this cDNA encodes a biologically active receptor by showing that human acidic and basic fibroblast growth factors activate this receptor as measured by Ca-45(2+) efflux assays. These data establish the existence of an additional member of the FGFR family that we have named FGFR-3.