Cobalt-Doped Zinc Oxide Nanoparticle-MoS2 Nanosheet Composites as Broad-Spectrum Bactericidal Agents
Cobalt-Doped Zinc Oxide Nanoparticle-MoS2 Nanosheet Composites as Broad-Spectrum Bactericidal Agents
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DOI：
10.1021/acsanm.0c02875
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发表时间：
2021-03-09
影响因子：
5.9
通讯作者：
Chen, Shaowei
中科院分区：
文献类型：
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作者：
Liu, Junli;Cheng, Wenxia;Chen, Shaowei
高性能抗菌剂的设计和工程对于对抗抗生素耐药性至关重要。在本研究中，开发了一种快速和广谱杀菌剂的基础上的纳米复合材料组成的钴掺杂氧化锌（CoZnO）纳米粒子和二硫化钼纳米片。采用化学沉淀法制备了CoZnO/MoS 2纳米复合材料。扫描和透射电子显微镜测量表明，CoZnO纳米粒子（约。直径为10 nm）聚集在MoS 2纳米片表面上，这有利于光生电子-空穴对的电荷分离，导致增强的光动力学抗微生物活性。在黑暗中的抗菌试验表明，在30 μ g的二硫化钼饲料（CoZnO/MoS 2 -30）制备的CoZnO/MoS 2纳米复合材料表现出最好的性能在一系列样品中，与最低抑制浓度为0.25，0.8和1.8毫克/毫升（-1）对革兰氏阴性细菌大肠杆菌，革兰氏阳性细菌金黄色葡萄球菌和真菌黄曲霉菌，分别。在光照条件下，抗菌性能显著增强，对大肠杆菌的灭活率达94.0%。大肠杆菌实现与20 μ g mL（-1）CoZnO/MoS 2 -30纳米复合材料下光照射（15 W，360 nm）5分钟。高抗菌活性可以归因于过氧化物酶样的光催化活性，这是有利于产生活性氧物种，证明在透射电子显微镜，电子自旋共振，和细胞内谷胱甘肽氧化测量。本研究的结果突出了CoZnO/MoS 2纳米复合材料作为有效的光动力抗菌剂的意义。
The design and engineering of high-performance antimicrobial agents is critical for combating antibiotic resistance. In the present study, a rapid and broad-spectrum bactericidal agent is developed based on nanocomposites consisting of cobalt-doped zinc oxide (CoZnO) nanoparticles and MoS2 nanosheets. The CoZnO/MoS2 nanocomposites are prepared by a facile chemical precipitation method at controlled CoZnO and MoS2 feeds. Scanning and transmission electron microscopic measurements show that CoZnO nanoparticles (ca. 10 nm in diameter) are clustered on the MoS2 nanosheet surface, which facilitates the charge separation of the photo-generated electron-hole pairs, leading to enhanced photodynamic antimicrobial activity. Antibacterial assays in the dark show that the CoZnO/MoS2 nanocomposite prepared at 30 mu g of MoS2 feed (CoZnO/MoS2-30) exhibits the best performance among a series of samples, with minimum inhibitory concentrations of 0.25, 0.8, and 1.8 mg mL(-1) toward the Gram-negative bacterium Escherichia coli, Gram-positive bacterium Staphylococcus aureus and fungus Aspergillus flavus, respectively. The antibacterial performance is markedly enhanced under photoirradiation, where 94.0% inactivation of E. coli is achieved with 20 mu g mL(-1) CoZnO/MoS2-30 nanocomposite under photoirradiation (15 W, 360 nm) for 5 min. The high antibacterial activity can be ascribed to peroxidase-like photocatalytic activity that is conducive to the generation of reactive oxygen species, as evidenced in transmission electron microscopy, electron spin resonance, and intracellular glutathione oxidation measurements. The results of the present study highlight the significance of CoZnO/MoS2 nanocomposites as potent photodynamic antibacterial agents.