Biosynthesis of the nucleoside antibiotic angustmycins: identification and characterization of the biosynthetic gene cluster reveal unprecedented dehydratase required for exo-glycal formation
Biosynthesis of the nucleoside antibiotic angustmycins: identification and characterization of the biosynthetic gene cluster reveal unprecedented dehydratase required for exo-glycal formation
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DOI：
10.1038/s41429-021-00466-7
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发表时间：
2021-08
期刊：
影响因子：
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通讯作者：
Taro Shiraishi;Jiaqi Xia;Teruhito Kato;T. Kuzuyama
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文献类型：
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作者：
Taro Shiraishi;Jiaqi Xia;Teruhito Kato;T. Kuzuyama
核苷类抗生素狭霉素由链霉菌属的一些菌株产生，由腺嘌呤和C6糖组成，具有抗菌和抗肿瘤活性。在这项研究中，我们提出了一个生物合成途径，利用异源表达实验再加上在电子分析的angustmycin生物合成基因（agm）集群。Agm6的生化特性表明其在狭霉素生物合成中作为一种前所未有的酶发挥作用。
The nucleoside antibiotic angustmycin, produced by someStreptomycesstrains, is composed of adenine and C6sugar and shows antibiotic and antitumor activities. In this study, we propose a biosynthetic pathway for angustmycin using a heterologous expression experiment coupled with in silico analysis of the angustmycin biosynthetic gene (agm) cluster. The biochemical characterization of Agm6 demonstrated its role in angustmycin biosynthesis as an unprecedented dehydratase.