Application of ergothioneine-rich extract from an edible mushroom Flammulina velutipes for melanosis prevention in shrimp, Penaeus monodon and Litopenaeus vannamei
Application of ergothioneine-rich extract from an edible mushroom Flammulina velutipes for melanosis prevention in shrimp, Penaeus monodon and Litopenaeus vannamei
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DOI：
10.1016/j.foodres.2011.10.030
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发表时间：
2012-01-01
影响因子：
8.1
通讯作者：
Ohshima, Toshiaki
中科院分区：
文献类型：
--
作者：
Encarnacion, Angel B.;Fagutao, Fernand;Ohshima, Toshiaki
研究了食用菌（Flammulinavelutipes）菌基和子实体废弃物中含有9.13 +/- 2.03 mg/mL 2-巯基-L-组氨酸-甜菜碱（ergothioneine，ESH）的水溶性粗提物对黑色素沉着的抑制作用。将活的完全生长的斑节对虾（Penaeus monodon）和太平洋白色对虾（Litopenaeus vannamei）浸泡在0.5%w/v的蘑菇提取物溶液中1 h，显著降低对虾血淋巴中多酚氧化酶（PPO）活性和血细胞中酚氧化酶原（proPO）基因的表达。因此，防止了在冰储存期间处理的虾中黑变病的发展。用0.05%w/v亚硫酸钠和4-己基-1，3-苯甲腈溶液处理具有类似的效果。酶活性测定表明ESH是一种非竞争性抑制剂。有人建议，ESH可能直接与铜2+在推定的结合位点的PPO和proPO，铜螯合活性分析的基础上，从而防止黑变病的虾。本研究表明，应用富ESH的F。从修剪废料中提取的金针菇提取物是合成黑色素沉着抑制剂的有效天然替代物，用于预防虾的死后黑色素沉着。(C)2011爱思唯尔有限公司版权所有。
This report investigated the melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom (Flammulina velutipes) containing 9.13 +/- 2.03 mg/mL 2-thiol-L-histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp (Penaeus monodon) and Pacific white shrimp (Litopenaeus vannamei) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase (proPO) gene in hemocytes. Consequently, the development of melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu2+ at the putative binding sites of PPO and proPO, based on copper-chelating activity analyses, thus preventing melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic melanosis-inhibiting agents to prevent postmortem melanosis in shrimp. (C) 2011 Elsevier Ltd. All rights reserved.