Characterization of flagellins isolated from a highly motile strain of Lactobacillus agilis.
Characterization of flagellins isolated from a highly motile strain of Lactobacillus agilis.
复制标题
DOI：
10.1186/s12866-016-0667-x
复制
发表时间：
2016-03-22
期刊：
影响因子：
4.2
通讯作者：
Okada S
中科院分区：
文献类型：
--
作者：
Kajikawa A;Midorikawa E;Masuda K;Kondo K;Irisawa T;Igimi S;Okada S
大多数乳酸菌是非运动性的，但有些是有鞭毛的，表现出运动性。到目前为止，对运动型乳杆菌的研究很少，对其鞭毛的特性也知之甚少。本研究以一株运动能力较强的Agilis乳杆菌为研究对象，对其鞭毛蛋白进行了转录分析和鉴定。与另一种肠道分离株瘤胃乳杆菌不同，Agilis菌株的鞭毛细丝可能由两个同源但不同的鞭毛组成。还观察了鞭毛细丝的糖基化以及它们对热、酸和十二烷基硫酸钠的耐受性。通过刺激Caco-2细胞评价鞭毛蛋白的免疫活性。结果表明，该蛋白的TLR5刺激活性减弱，可能是由于TLR5识别位点不完整所致。Agilis BKN88的鞭毛细丝由两个同源的糖基化鞭毛蛋白组成，可能有一个不完整的TLR5识别位点。鞭毛的特性可能是作为胃肠道共生微生物适应的结果。
Most lactic acid bacteria are non-motile but some of them are flagellated and exhibit motility. So far, motile lactobacilli have rarely been studied, and characteristics of their flagellins are poorly understood. In this study, a highly motile strain of Lactobacillus agilis was recruited for transcriptional analysis and characterization of its flagellins. Unlike another motile lactic acid bacteria of intestinal isolate, Lactobacillus ruminis, flagellar filaments of the L. agilis strain probably consist of two homologous but distinct flagellins. Glycosylation of the flagellar filaments and their resistance to heat, acid and SDS were also observed. The immunological activity of the flagellins was evaluated through the stimulation of Caco-2 cells. The results show that TLR5-stimulating activity of the protein is attenuated, likely due to an incomplete TLR5-recognition site. The flagella filaments of L. agilis BKN88 consist of two homologous glycosylated flagellins, which likely have an incomplete TLR5-recognition site. The characteristics of the flagellin are presumably a consequence of adaptation as a commensal microbe in the gastrointestinal tract.