A plant virus enhances odorant-binding protein 5 (OBP5) in the vector whitefly for more actively olfactory orientation to the host plant
A plant virus enhances odorant-binding protein 5 (OBP5) in the vector whitefly for more actively olfactory orientation to the host plant
复制标题
植物病毒增强了烟粉虱载体中的气味结合蛋白 5 (OBP5)，从而更积极地嗅觉到宿主植物
DOI：
10.1002/ps.7313
复制
发表时间：
2022-12-22
影响因子：
4.1
通讯作者：
Yan, Fengming
中科院分区：
文献类型：
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作者：
He, Haifang;Li, Jingjing;Yan, Fengming
背景粉虱烟粉虱（半翅目：粉虱科）是一种臭名昭著的农业害虫，也是全世界许多植物病毒的有效载体。葫芦褪绿黄化病毒（CCYV）仅由烟粉虱以半持久性方式传播，是许多国家葫芦作物的严重致病因子。植物病毒可以操纵昆虫媒介的行为来促进自身传播，但其潜在机制仍不清楚。结果在这项研究中，我们的观察表明，携带 CCYV 的烟粉虱更积极地针对宿主植物黄瓜。转录组分析和定量聚合酶链反应与逆转录分析表明，气味结合蛋白 5 (OBP5) 随着病毒获取而上调。序列和系统发育分析表明BtabOBP5与其他半翅目昆虫的9个OBP高度同源。此外，OBP5沉默的粉虱显着改变了它们对黄瓜植物和黄瓜释放的一些典型挥发性有机化合物的定向行为。结论本研究描述了一种新机制，通过半持久性植物病毒可以调节媒介昆虫的嗅觉系统，从而影响昆虫的嗅觉行为以及与寄主植物的关系。这些结果为未来开发潜在的基于嗅觉的害虫管理策略提供了基础。 (c) 2022 作者。 《害虫管理科学》由 John Wiley & Sons Ltd 代表化学工业协会出版。
BACKGROUNDThe whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a notorious agricultural pest and the effective vector of many plant viruses worldwide. Cucurbit chlorotic yellows virus (CCYV), exclusively transmitted by B. tabaci in a semipersistent manner, is a serious causal agent in cucurbit crops in many countries. Plant viruses can manipulate the behaviors of insect vectors to promote the spread of themselves, but underlying mechanisms are remaining unclear. RESULTSIn this study, our observations indicated that B. tabaci, when carrying CCYV, oriented more actively to the host plant cucumber. Transcriptome analysis and quantitative polymerase chain reaction with reverse transcription analysis showed that the odorant-binding protein 5 (OBP5) was upregulated with viral acquisition. Sequence and phylogenetic analysis showed that BtabOBP5 was highly homologous with nine OBPs from other hemipteran insects. In addition, OBP5-silenced whiteflies significantly altered their orientation behavior towards cucumber plants and towards some typical volatile organic compounds released from cucumbers. CONCLUSIONThis study described a novel mechanism by which the olfactory system of vector insects could be regulated by a semipersistent plant virus, thereby affecting insect olfactory behavior and relationship with host plants. These results provided a basis for developing potential olfaction-based pest management strategies in the future. (c) 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.