CHARACTERIZATION OF A PHOSPHATE-ACCUMULATOR MUTANT OF ARABIDOPSIS-THALIANA
CHARACTERIZATION OF A PHOSPHATE-ACCUMULATOR MUTANT OF ARABIDOPSIS-THALIANA
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DOI：
10.1104/pp.107.1.207
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发表时间：
1995-01-01
期刊：
影响因子：
7.4
通讯作者：
RANDALL, PJ
中科院分区：
文献类型：
--
作者：
DELHAIZE, E;RANDALL, PJ
我们已经鉴定了拟南芥的一个新的突变，该突变位于一个名为Pho2的基因座上。与野生型幼苗相比，ph2突变体在叶片中积累的全磷(主要是无机磷)增加了3倍。此外，我们还分离到了一个叶片中磷浓度较低的突变体(位置命名为Pho1-2，由Y.Poirier，S.Thoma，C.Somerville，J.Schiefelbein[1991]Plants Physiol 97：1087-1093描述)。在高蒸腾条件下，Pho2幼苗叶片严重磷中毒，而Pho1-2突变体植株地上部缺磷，野生型幼苗正常。在F-2代中发现了一个由单突变体间杂交产生的Pho1/Pho2双突变体，并表现为Pho1表型。在出现磷毒害症状之前，磷是ph2突变体中唯一浓度高于野生型幼苗的矿质养分。与野生型相比，Pho2突变体在茎、角果和种子中具有更高的PI浓度，而根中的PI浓度与Pho1突变体或野生型幼苗相似或更低。我们认为，Pho2突变影响了拟南芥枝条中通常参与调节PI浓度的功能。
We have characterized a novel mutation of Arabidopsis thaliana at a locus designated pho2. pho2 mutants accumulated up to 3-fold more total P in leaves, mostly as inorganic phosphate (Pi), than wild-type seedlings. In addition, we isolated a mutant (locus designated pho1-2, an allelle of pho1-1 described by Y. Poirier, S. Thoma, C. Somerville, J. Schiefelbein [1991] Plant Physiol 97: 1087-1093) with low Pi concentrations in leaves. When grown under high transpiration conditions, leaves of pho2 seedlings became severely P intoxicated, whereas shoots of pho1-2 mutants were P deficient and wild-type seedlings were normal. A pho1/pho2 double mutant resulting from a cross between the single mutants was identified in the F-2 generation and shown to have a pho1 phenotype. Prior to the development of P toxicity symptoms, P was the only mineral nutrient whose concentration was greater in pho2 mutants than wildtype seedlings. Compared to wild-type, pho2 mutants had greater Pi concentrations in stems, siliques, and seeds, but roots of pho2 mutants had similar or lower Pi concentrations than either pho1 mutants or wild-type seedlings. We suggest that the pho2 mutation affects a function normally involved in regulating the concentration of Pi in shoots of Arabidopsis.