Qidantongmai protects endothelial cells against hypoxia-induced damage through regulating the serum VEGF-a level.
Qidantongmai protects endothelial cells against hypoxia-induced damage through regulating the serum VEGF-a level.
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DOI：
10.4314/ajtcam.v9i2.5
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
B. Wang;Wen Wang;Feng Li;Zong-ren Wang;Jing Ma;Gang Zhao
中科院分区：
文献类型：
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作者：
B. Wang;Wen Wang;Feng Li;Zong-ren Wang;Jing Ma;Gang Zhao
芪丹通脉（QDTM）是一种中药制剂，长期以来被民间医学用于治疗心血管疾病。然而，人们对其潜在机制知之甚少。本研究旨在确定QDTM在体外和体内缺氧条件下对内皮细胞的影响。分离原代人脐静脉内皮细胞（HUVECs），用含QDTM的血清或生理盐水对照进行预处理，然后在低氧（2%氧）下培养24 h。Sprague-Dawley大鼠给予1 ml/100 g QDTM或生理盐水，每天2次，连续培养4 d，并进行缺氧（6小时/天，间断缺氧，360 mm Hg）处理。QDTM不仅能在体外显著维持huvec细胞活力（P < 0.05），降低细胞凋亡（P < 0.05），保护huvec免受缺氧损伤，还能在体内保护肝内皮细胞免受缺氧损伤。且对缺氧7 d的大鼠血清VEGF-A水平有升高作用（P < 0.05），对缺氧14 d的大鼠血清VEGF-A水平有抑制作用。QDTM是一种有效的制剂，可以保护内皮细胞免受缺氧引起的损伤。QDTM调节血清VEGF-A水平的能力可能在其对内皮细胞的作用中起重要作用。
Qidantongmai (QDTM) is a Traditional Chinese Medicine (TCM) preparation that has long been used in folk medicine for the treatment of cardiovascular diseases. However, the underlying mechanisms are poorly understood. The present study was designed to determine the effects of QDTM on endothelial cells under hypoxic conditions both in vitro and in vivo. Primary human umbilical vein endothelial cells (HUVECs) were isolated, pretreated with QDTM medicated serum or saline control, and then cultured under hypoxia (2% oxygen) for 24 h. Sprague-Dawley rats were administered 1 ml/100 g of QDTM or saline twice a day for 4 days and treated with hypoxia (6 hours/day, discontinuous hypoxia, 360 mm Hg). QDTM not only protected HUVECs from hypoxia-induced damage by significantly retaining cell viability (P < 0.05) and decreasing apoptosis (P < 0.05) in vitro, but also protected liver endothelial cells from hypoxia-induced damage in vivo. Moreover, QDTM increased the serum VEGF-A level (P < 0.05) in rats treated with hypoxia for 7 days but suppressed the upregulation of serum VEGF-A in rats treated with hypoxia for 14 days. QDTM is a potent preparation that can protect endothelial cells against hypoxia-induced damage. The ability of QDTM to modulate the serum VEGF-A level may play an important role in its effects on endothelial cells.