INVOLVEMENT OF ACTIVATOR PROTEIN IN THE ACTIVATION OF TRYPTOPHAN-HYDROXYLASE BY CAMP-DEPENDENT PROTEIN-KINASE
INVOLVEMENT OF ACTIVATOR PROTEIN IN THE ACTIVATION OF TRYPTOPHAN-HYDROXYLASE BY CAMP-DEPENDENT PROTEIN-KINASE
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DOI：
10.1016/0014-5793(90)81004-8
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发表时间：
1990-07-30
期刊：
影响因子：
3.5
通讯作者：
FUJISAWA, H
中科院分区：
文献类型：
--
作者：
MAKITA, Y;OKUNO, S;FUJISAWA, H
在蛋白质磷酸化条件下，与cAMP类似物孵育可使大鼠脑干粗提物的色氨酸羟基酶活性提高约2倍。CAMP依赖的酶激活过程不仅需要cAMP依赖的蛋白激酶，还需要激活蛋白。CAMP依赖的蛋白激酶激活的酶的动力学性质与钙调素依赖的蛋白激酶II激活的酶非常相似。
The tryptophan hydroxylase activity of the crude extract from rat brain stem was stimulated approximately 2-fold by incubation with cAMP analogues under protein phosphorylating conditions. The cAMP-dependent activation process of the enzyme needed not only cAMP-dependent protein kinase but also activator protein. The kinetic properties of the enzyme activated by cAMP-dependent protein kinase were very similar to those of the enzyme activated by calmodulin-dependent protein kinase II.