Prognostic value of SATB2 expression in patients with esophageal squamous cell carcinoma.
Prognostic value of SATB2 expression in patients with esophageal squamous cell carcinoma.
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SATB2表达在食管鳞癌患者中的预后价值
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Jiang Jie
中科院分区：
文献类型：
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作者：
Luo Qi-Cong;Xie Jing-Dun;Fa Xian-En;Jiang Jie
SATB2是一种特殊的富含at结合蛋白家族的成员，已被证明影响许多肿瘤的发生。然而，SATB2在食管鳞状细胞癌（ESCC）中的作用尚不清楚。本研究采用实时定量逆转录聚合酶链反应（qRT-PCR）、Western blotting、免疫组化等方法检测ESCC组织及癌旁非癌组织中SATB2 mRNA和蛋白水平的表达。统计分析SATB2表达与临床病理因素及预后的关系。Western blotting和qRT-PCR结果显示，SATB2组织中SATB2 mRNA和蛋白的表达水平均显著低于非癌组织。免疫组化分析显示，SATB2表达与临床分期和组织学分化有显著相关性。Kaplan-Meier分析结果显示，SATB2低表达导致ESCC患者预后明显不良。重要的是，多变量分析显示，低SATB2表达是ESCC患者的独立预后因素。综上所述，我们的数据表明SATB2在ESCC的进展中起着重要的作用，肿瘤组织中SATB2表达的降低可以作为ESCC患者的潜在预后指标。
SATB2, a member of the family of special AT-rich binding proteins, has been shown to affect numerous tumorigenesis. However, the role of SATB2 in esophageal squamous cell carcinoma (ESCC) remains unclear. In this study, the SATB2 expression was examined at mRNA and protein levels by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR), Western blotting, and immunohistochemistry in ESCC tissues and adjacent non-cancerous tissues. Statistical analyses were applied to test the associations between SATB2 expression, clinicopathologic factors, and prognosis. Western blotting and qRT-PCR showed that the expression levels of SATB2 mRNA and protein were both significantly lower in SATB2 tissues than those in non-cancerous tissues. Immunohistochemistry analysis showed that SATB2 expression was significantly correlated with clinical stage and Histological differentiation. The results of Kaplan-Meier analysis indicated that a low expression level of SATB2 resulted in a significantly poor prognosis of ESCC patients. Importantly, multivariate analysis showed that low SATB2 expression was an independent prognostic factor for ESCC patients. In sum, our data suggest that SATB2 plays an important role in ESCC progression, and that decreased expression of SATB2 in tumor tissues could be used as a potential prognostic marker for patients with ESCC.