Radioprotection of hematopoietic stem cells by interleukin-1.
Radioprotection of hematopoietic stem cells by interleukin-1.
复制标题
DOI：
10.1182/blood.v81.3.639.639
复制
发表时间：
1993-02
影响因子：
2.6
通讯作者：
Zucali;J. Moreb;W. Gibbons;J. Alderman;A. Suresh;Y. Zhang;B. Shelby
中科院分区：
文献类型：
--
作者：
Zucali;J. Moreb;W. Gibbons;J. Alderman;A. Suresh;Y. Zhang;B. Shelby
辐射防护剂，如白细胞介素1(IL-1)和肿瘤坏死因子(肿瘤坏死因子-α)，在照射前给予，可保护动物免受辐射损伤。然而，在体内应用这些细胞因子并不能确定这些保护作用是否直接作用于造血系统。在本研究中，我们让男性骨髓细胞在照射和骨髓移植前接受IL-1的体外处理。我们发现，与未经处理的照射骨髓细胞相比，照射前用IL-1预处理的雄性骨髓细胞提高了接受照射的雌性受者小鼠的存活率。此外，接受IL-1处理的男性供者骨髓细胞的照射女性受者在移植后长达3个月的时间里，其骨髓、脾和胸腺中男性供者细胞的存在增加。此外，一系列移植研究表明，只有接受了IL-1处理细胞移植的小鼠骨髓的第三代女性受者才能检测到男性细胞。这些结果表明，IL-1预处理可以保护短期和长期再生干细胞免受辐射损伤，并且这些干细胞能够重建受者的髓系和淋巴系器官。
Radioprotective agents such as interleukin-1 (IL-1) and tumor necrosis factor (TNF-alpha), when given prior to irradiation, protect animals from radiation damage. However, in vivo administration of these cytokines does not allow one to determine whether the protective effects act directly on the hematopoietic system. In the present study, we subjected male bone marrow cells to in vitro treatment with IL-1 prior to irradiation and bone marrow transplantation. We found that male bone marrow cells pretreated with IL-1 prior to irradiation increased the survival of irradiated female recipient mice when compared with nontreated irradiated marrow cells. In addition, irradiated female recipients that received IL-1-pretreated male donor bone marrow cells displayed an increased presence of male donor cells in their bone marrow, spleen, and thymus for up to 3 months posttransplant. Furthermore, serial transplantation studies revealed that male cells could only be detected in tertiary female recipients who received bone marrow from mice transplanted with IL-1-treated cells. These results indicate that IL-1 pretreatment protects both short-term and long-term repopulating stem cells from an irradiation insult and that these cells are capable of reconstituting the myeloid and lymphoid organs of recipient mice.