Premature progression of anther early developmental programs accompanied by comprehensive alterations in transcription during high-temperature injury in barley plants
Premature progression of anther early developmental programs accompanied by comprehensive alterations in transcription during high-temperature injury in barley plants
复制标题
DOI：
10.1007/s00438-007-0229-x
复制
发表时间：
2007-07-01
影响因子：
3.1
通讯作者：
Higashitani, Atsushi
中科院分区：
文献类型：
--
作者：
Oshino, Takeshi;Abiko, Mafumi;Higashitani, Atsushi
高温胁迫导致许多植物雄性生殖发育失育。在这里，我们报道了大麦植物（Hordeum vulgare L）花药早期发育过程中高温伤害的推测机制。在高温条件下（白天30℃/晚上25℃），花药细胞增殖停滞，空泡化增加，叶绿体过度发育，线粒体、核膜和粗内质网（RER）出现一定程度的异常，胚珠细胞未见异常。此外，花粉母细胞中绒毡层细胞的过早降解和减数分裂前期的过早进展也被观察到。为了监测高温损伤期间的转录变化，我们使用22K Barley1基因芯片进行了DNA微阵列分析。在高温处理或不高温处理的情况下，在穗粒早期发育的四个时间点和作为对照的幼苗营养生长期间捕获了表达谱。与穗部相比，非生物或生物胁迫相关基因在高温下的幼苗中同样或更显著地上调。相反，与组蛋白、DNA复制起始、线粒体和核糖体相关的某些基因在暴露的穗中被特异性抑制。原位杂交研究表明，高温下发育中的花药细胞发生局部抑制。微阵列分析还表明，包括减数分裂特异性基因Asy1和花药特异性脂质转移蛋白基因在内的一系列基因在高温条件下早期过早上调。实时定量RT-PCR分析很好地证实了DNA芯片预测的某些关键基因的表达差异。这些结果表明，高温导致花药早期发育程序和命运的提前进展，如PMCs向减数分裂的进展、细胞增殖阻滞和花药壁细胞的降解，并伴随转录的全面改变。
High-temperature stress causes abortive male reproductive development in many plant species. Here, we report a putative mechanism of high-temperature injury during anther early development in barley plants (Hordeum vulgare L). Under high-temperature conditions (30 degrees C day/25 degrees C night), cell-proliferation arrest, increased vacuolization, over-development of chloroplasts, and certain abnormalities of the mitochondria, nuclear membrane, and rough endoplasmic reticulum (RER) were observed in developing anther cells, but not in developing ovule cells. Moreover, premature degradation of tapetum cells and premature progression to meiotic prophase in pollen mother cells (PMCs) were also observed. To monitor transcriptional alterations during high-temperature injury, we performed DNA microarray analysis using the 22K Barley1 GeneChip. Expression profiles were captured at four time points during the early development of panicles, and during vegetative growth of seedlings as a control, with or without high-temperature treatment. Abiotic or biotic stress related genes were equally or more dominantly up-regulated in the seedlings exposed to high temperatures compared with the panicles. In contrast, certain genes associated with histones, DNA replication initiation, mitochondria, and ribosomes were specifically repressed in the exposed panicles. In situ hybridization studies indicated that repression locally occurred on the developing anther cells exposed to high temperatures. Microarray analysis also indicated that a series of genes, including a meiosis-specific gene Asy1 and anther-specific lipid transfer protein genes, was prematurely up-regulated at an earlier stage under high-temperature conditions. Real-time quantitative RT-PCR analyses well confirmed the expression differences of certain key genes predicted by the DNA microarrays. These results suggest that high-temperature causes premature progression of anther early development program and fate, such as progression to meiosis of PMCs, cell-proliferation arrest and degradation in anther wall cells, accompanied by comprehensive alterations in transcription.