FEULGEN DNA STAINABILITY OF BONE-TUMORS AFTER DEMINERALIZATION
FEULGEN DNA STAINABILITY OF BONE-TUMORS AFTER DEMINERALIZATION
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DOI：
10.1002/cyto.990080610
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发表时间：
1987-11-01
期刊：
影响因子：
--
通讯作者：
KREICBERGS, A
中科院分区：
文献类型：
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作者：
BAUER, HCF;KREICBERGS, A
显微分光光度法DNA分析档案骨肿瘤组织往往阻碍了以前的酸脱矿，破坏Feulgen DNA酶。为了找到一种替代酸的前瞻性DNA研究的骨肿瘤组织切片，Feulgen染色新鲜骨肉瘤标本脱矿后，在中性EDTA进行了研究。还研究了档案组织的弱Feulgen染色切片的DNA分析的可靠性。与非脱矿制剂相比，EDTA中的四个新鲜标本的脱矿作用略微降低了Feulgen DNA的含量，但不影响倍性水平的测定。一个二倍体和一个超倍体骨肉瘤的水解试验表明，对照和肿瘤细胞之间的染色关系没有改变EDTA预处理。对于需要脱矿的骨肿瘤的DNA研究，EDTA提供了一种保留核Feulgen的方法。在22个档案骨肉瘤标本的不同Feulgen染色，三个不同的上限的光传输（75，85，和95%）被应用到测试的意义，背景干扰核染色强度。对照组和肿瘤细胞群的中位完全消退之间的关系并没有受到改变透射上限的显著影响，除了4个染色不良的病变。这四个样本的对照细胞显示出的中位总消光小于所遇到的最大值的三分之一。结果表明，弱染色的档案标本可以进行测试，以选择那些适合的倍性测定。
Microspectrophotometric DNA analysis of archival bone tumor tissue is often impeded by previous acid demineralization, which destroys Feulgen DNA stainability. To find an alternative to acid for prospective DNA studies of bone tumors in tissue sections, Feulgen stainability of fresh osteosarcoma specimens after demineralization in neutral EDTA was investigated. The reliability of DNA analysis of weakly Feulgen-stained sections from archival tissue was also studied. Demineralization of four fresh specimens in EDTA slightly reduced Feulgen DNA stainability compared to nondemineralized preparations but did not affect the determination of ploidy level. Hydrolysis tests of one diploid and one hyperploid osteosarcoma showed that the staining relationship between control and tumor cells was not altered by EDTA pretreatment. For DNA studies of bone tumors requiring demineralization, EDTA offers a means of retaining nuclear Feulgen stainability. In 22 archival osteosarcoma specimens of varying Feulgen stainability, three different upper limits of light transmission (75, 85, and 95%) were applied to test the significance of background disturbances in relation to nuclear stain intensity. The relationship between the median total extinction of the control and tumor cell populations was not significantly affected by altering the upper transmission limit except in four poorly stained lesions. The control cells of these four specimens exhibited a median total extinction less than one-third of the maximum encountered. The results suggest that weakly stained archival specimens can be tested for selecting those appropriate for ploidy determination.