Interhomolog recombination and loss of heterozygosity in wild-type and Bloom syndrome helicase (BLM)-deficient mammalian cells
Interhomolog recombination and loss of heterozygosity in wild-type and Bloom syndrome helicase (BLM)-deficient mammalian cells
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DOI：
10.1073/pnas.1104421108
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发表时间：
2011-07-19
影响因子：
11.1
通讯作者：
Jasin, Maria
中科院分区：
文献类型：
--
作者：
LaRocque, Jeannine R.;Stark, Jeremy M.;Jasin, Maria
基因组完整性在肿瘤细胞中经常受到损害，如遗传改变导致杂合性丧失（LOH）所示。LOH的一种机制是同源染色体之间的有丝分裂交叉重组，可能由双链断裂（DSB）启动。为了研究LOH与dsb诱导的同源间重组的关系，我们使用报告基因分析了来自F1杂交胚胎的小鼠胚胎干细胞的重组事件。在这项研究中，我们能够确定LOH事件，尽管它们在野生型细胞中很少发生(
Genomic integrity often is compromised in tumor cells, as illustrated by genetic alterations leading to loss of heterozygosity (LOH). One mechanism of LOH is mitotic crossover recombination between homologous chromosomes, potentially initiated by a double-strand break (DSB). To examine LOH associated with DSB-induced interhomolog recombination, we analyzed recombination events using a reporter in mouse embryonic stem cells derived from F1 hybrid embryos. In this study, we were able to identify LOH events although they occur only rarely in wild-type cells (