Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata.
Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata.
复制标题
用于分析细胞骨架和胞间连丝其他调节因子的细胞间连接测定。
DOI：
10.1007/978-1-0716-2867-6_14
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Barr Z
中科院分区：
文献类型：
--
作者：
Barr Z
肌动蛋白细胞骨架与植物细胞间连丝（plasmodesmata）有着密切的联系，但迄今尚未完全了解。胞间连丝是植物发育和对生物和非生物胁迫的反应所必需的，并且促进代谢物和激素以及大分子如蛋白质和RNA的细胞间交换。胞间连丝的分子大小排阻限制是动态调节的，包括肌动蛋白相关蛋白。因此，对胞间连丝调控的实验分析可以为植物细胞骨架的研究提供参考。本章介绍了两个简单的基于成像的协议，用于分析叶片中的大分子细胞与细胞的连接。
The actin cytoskeleton has close but so far incompletely understood connections to plasmodesmata, the cell junctions of plants. Plasmodesmata are essential for plant development and responses to biotic and abiotic stresses and facilitate the intercellular exchange of metabolites and hormones but also macromolecules such as proteins and RNAs. The molecular size exclusion limited of plasmodesmata is dynamically regulated, including by actin-associated proteins. Therefore, experimental analysis of plasmodesmal regulation can be relevant to plant cytoskeleton research. This chapter presents two simple imaging-based protocols for analyzing macromolecular cell-to-cell connectivity in leaves.