Biosynthesis of antibiotic prodiginines in the marine bacterium Hahella chejuensis KCTC 2396
Biosynthesis of antibiotic prodiginines in the marine bacterium Hahella chejuensis KCTC 2396
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DOI：
10.1111/j.1365-2672.2006.03172.x
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发表时间：
2007-04-01
影响因子：
4
通讯作者：
Lee, C. H.
中科院分区：
文献类型：
--
作者：
Kim, D.;Lee, J. S.;Lee, C. H.
目的：济州石螺（Hahella chejuensis KCTC 2396）产生红色色素，具有抗菌和杀藻活性。色素的主要红色代谢产物被鉴定为抗生素灵菌红素。本研究的目的是在KCTC 2396中发现新的抗生素灵菌红素类似物，并分析灵菌红素的生物合成模式。方法和结果：除灵菌红素外，其他红色素成分均为已知的二吡咯二吡咯亚甲基灵菌红素、去甲灵菌红素和十一烷基灵菌红素。此外，四个新的灵菌红素类似物，其中每一个是区别于灵菌红素（C-5），根据烷基链长度（C-3-C-7）的差异，检测到少量的液相色谱质谱/质谱。由于存在的细胞毒性甲氧基，预计所有新的灵菌红素类似物是biological.Conclusions：四个特征的灵菌红素，包括灵菌红素和四个新的灵菌红素类似物在KCTC 2396中以不同的比例产生。所有的Prodiginines具有一个共同的线性三吡咯基结构和一个细胞毒性的methoxy group.Significance和影响的研究：这项研究表明，KCTC 2396是第一次能够产生抗生素灵菌红素，十一烷基灵菌红素和新的灵菌红素类似物的混合物中的色素。它还表明，KCTC 2396拥有一个新的系统，在一个单一的微生物中同时生产多种前体素。
Aims: Hahella chejuensis KCTC 2396 produces red pigments, showing antibacterial and algicidal activities. The main red-coloured metabolite of the pigments was identified as antibiotic prodigiosin. With the expectation that the red pigments are a mixture of a series of close relatives, the aim of the present study is to detect new antibiotic prodigiosin analogues and to analyse the biosynthetic pattern for prodiginines in KCTC 2396.Methods and Results: Except prodigiosin, the other constituents in the red pigments were confirmed as well-known dipyrrolyldipyrromethene prodigiosin, norprodigiosin, and undecylprodiginine. Additionally, four new prodigiosin analogues, each of which was distinguished from prodigiosin (C-5), according to differences in alkyl chain length (C-3-C-7), were detected in small quantities by liquid chromatography mass spectrometry/mass spectrometry spectroscopy. Owing to the presence of a cytotoxic methoxy group, it is expected that all the new prodigiosin analogues are bioactive.Conclusions: Four characterized prodiginines, including prodigiosin and four new prodigiosin analogues are produced in different ratio in KCTC 2396. All of the prodiginines possess a common linear tripyrrolyl structure and a cytotoxic methoxy group.Significance and Impact of the Study: This study shows for the first time that KCTC 2396 is able to produce antibiotic prodigiosin, undecylprodiginine and new prodigiosin analogues in a mixture of pigments. It is also shown that KCTC 2396 possesses a novel system for the simultaneous production of multiple prodiginines in a single micro-organism.