A BACULOVIRUS BLOCKS INSECT MOLTING BY PRODUCING ECDYSTEROID UDP-GLUCOSYL TRANSFERASE
A BACULOVIRUS BLOCKS INSECT MOLTING BY PRODUCING ECDYSTEROID UDP-GLUCOSYL TRANSFERASE
复制标题
DOI：
10.1126/science.2505387
复制
发表时间：
1989-09-08
期刊：
影响因子：
56.9
通讯作者：
MILLER, LK
中科院分区：
文献类型：
--
作者：
OREILLY, DR;MILLER, LK
新近发现的昆虫杆状病毒Autograph a calfornica核型多角体病毒基因的氨基酸序列与几种尿苷二磷酸葡萄糖醛酸基转移酶和至少一种UDP葡萄糖转移酶相似。遗传和生化研究证实，该基因编码蜕皮激素UDP-葡萄糖基转移酶(EGT)。这种酶催化葡萄糖从UDP-葡萄糖转移到蜕皮激素，蜕皮激素是昆虫的蜕皮激素。Egt基因的表达使病毒干扰了昆虫的正常发育，从而阻止了受感染的秋季粘虫幼虫的蜕皮。
The predicted amino acid sequence of a newly identified gene of the insect baculovirus Autographa californica nuclear polyhedrosis virus was similar to several uridine 5''-diphosphate (UDP)-glucuronosyl transferases and at least one UDP-glucosyl transferase. Genetic and biochemical studies confirmed that this gene encodes an ecdysteroid UDP-glucosyl transferase (egt). This enzyme catalyzes the transfer of glucose from UDP-glucose to ecdysteroids, which are insect molting hormones. Expression of the egt gene allowed the virus to interfere with normal insect development so that molting was blocked in infected larvae of fall armyworm (Spodoptera frugiperda).