Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae
Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae
复制标题
DOI：
10.1007/s10592-005-5477-x
复制
发表时间：
2006-02-01
影响因子：
2.2
通讯作者：
Severinghaus, LL
中科院分区：
文献类型：
--
作者：
Hsu, YC;Li, SH;Severinghaus, LL
本研究从兰屿角鸮（Otus elegans botelensis）的富集部分文库中开发了6个新的含四核苷酸重复基序（加塔/CTAT）的微卫星标记。这些位点均为多态性位点，符合Hardy-Weinberg平衡。我们跨物种测试这些和其他12个微卫星引物对先前开发的O。elegans对其他四种猫头鹰（O. lettia、山核桃O. spilocephalus、O. scops和Ninox scutulata）。结果表明，随着与O.优雅的。在3种大耳鸮中，大多数位点（66.7%、83.3%和100%）具有多态性，而在北鸮中只有5个位点（27.8%）具有多态性。具鳞片的。这些微卫星应该是非常有用的遗传标记，在研究交配系统，群体遗传学，和保护其他研究很少的旧大陆猫头鹰。
We developed six new microsatellite markers containing tetranucleotide repeat motifs (GATA/CTAT) for Lanyu scops owl (Otus elegans botelensis) from an enriched partial library. All these loci are polymorphic and conform to Hardy-Weinberg equilibrium. We cross-species tested these and 12 other microsatellite primer pairs previously developed from O. elegans on four other species of owls (O. lettia, O. spilocephalus, O. scops, and Ninox scutulata). Results showed that the degree of polymorphism decreased with increasing phylogenetic distance to O. elegans. Most loci (66.7, 83.3, and 100%) were polymorphic in the three Otus owls but only five (27.8%) were polymorphic in N. scutulata. These microsatellites should be very useful genetic markers in studying the mating system, population genetics, and conservation of other little studied Old World Otus owls.