Transcription. Response to Comment on "Mechanism of eukaryotic RNA polymerase III transcription termination".
Transcription. Response to Comment on "Mechanism of eukaryotic RNA polymerase III transcription termination".
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转录。对“真核RNA聚合酶III转录终止的机制的评论”。
DOI：
10.1126/science.1254246
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发表时间：
2014-08-01
期刊：
影响因子：
--
通讯作者：
Zenkin N
中科院分区：
文献类型：
--
作者：
Nielsen S;Zenkin N
Arimbasseri 等人在他们的评论中建议，为了终止体内转录，RNA 聚合酶 III 使用发夹依赖性终止以外的机制，并且纯化的聚合酶的特性可能取决于制备程序。证据表明我们的准备工作确实与其他方法不同。我们的新数据表明，除了发夹依赖性终止之外，细胞中可能还存在一种或多种“自动防故障”终止机制。
Arimbasseri et al., in their Comment, suggest that to terminate transcription in vivo, RNA polymerase III uses a mechanism other than hairpin-dependent termination and that properties of purified polymerase may depend on preparation procedure. Evidence suggests that our preparation is indeed different from that of other methods. Our new data suggest that, apart from hairpin-dependent termination, one or more “fail-safe” termination mechanisms may exist in the cell.