Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes
Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes
复制标题
使用内部产生的粗酶有效制备用于纤维素酶生产和玉米秸秆糖化的可溶性诱导剂
DOI：
10.1016/j.bej.2021.108296
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发表时间：
2022-01-01
影响因子：
3.9
通讯作者：
Zhao, Xinqing
中科院分区：
文献类型：
--
作者：
Li, Yonghao;Zhang, Peng;Zhao, Xinqing
尽管里氏木霉在生物精炼中利用木质纤维生物质生产高水平纤维素酶已经得到了广泛的研究，但为了降低生产成本，还需要更高效、更经济的水解法。本研究以高表达β-葡萄糖苷酶基因的重组里氏木霉PB-3为出发菌株，以葡萄糖为唯一底物，采用补料分批发酵，获得高达78.09 cBU/mL的纤维二糖酶活。将得到的粗纤维二糖酶用于转糖基化反应，动力学研究表明，反应需要高浓度的葡萄糖作为底物。我们发现，在使用优化的条件时，转糖基化时间(2 H)比通常报道的(72 H)要短得多。随后，将里氏木霉PB-3菌株的粗纤维二糖酶与里氏木霉Rut C30产生的粗纤维素酶混合用于玉米秸秆的水解率，得到了高达97.08%的产糖率。这项工作中开发的策略有利于通过内部生产纤维素酶来降低纤维素酶的生产成本，包括诱导剂制备和生物质的水解。
Although high-level cellulase production by Trichoderma reesei from lignocellulosic biomass in biorefinery has been widely studied, more efficient and economic hydrolysis is required to reduce the production cost. In this study, the recombinant T. reesei PB-3 strain overexpressing the beta-glucosidase gene was used to achieve a high cellobiase activity of 78.09 CBU/mL using glucose as the sole substrate using fed-batch fermentation. The obtained crude cellobiase was then used for transglycosylation, and kinetic studies showed that high concentration of glucose as the substrate is required for the reaction. We found that remarkably shorter transglycosylation time (2 h) than that was commonly reported (72 h), was sufficient when using the optimised conditions. Subsequently, the crude cellobiase from T. reesei PB-3 strain was also used to blend with the crude cellulase produced by T. reesei Rut C30 for hydrolysing corn stover, and a high glucose yield (97.08%) was achieved. The strategy developed in this work benefits reduction of cellulase production cost through in-house cellulase production for both inducer preparation and hydrolysis of the biomass.