Curcumin attenuated acute Propionibacterium acnes-induced liver injury through inhibition of HMGB1 expression in mice
Curcumin attenuated acute Propionibacterium acnes-induced liver injury through inhibition of HMGB1 expression in mice
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姜黄素通过抑制小鼠 HMGB1 表达减轻痤疮丙酸杆菌引起的急性肝损伤
DOI：
10.1016/j.intimp.2014.12.005
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发表时间：
2015-02-01
影响因子：
5.6
通讯作者：
Yang, Huilin
中科院分区：
文献类型：
--
作者：
Gu, Qiaoli;Guan, Honggeng;Yang, Huilin
姜黄素是从姜黄根茎中分离出来的酚类产品，对炎症性疾病具有保护作用。在这里，我们研究了姜黄素对痤疮丙酸杆菌（P.acnes）引起的急性炎症性肝损伤的保护作用。 C57BL/6 小鼠用痤疮丙酸杆菌引发，然后进行 LPS 攻击以诱导暴发性肝炎。从痤疮丙酸杆菌引发前 2 天开始，每天一次通过强饲法口服给予姜黄素或媒介物对照。我们发现姜黄素显着降低了小鼠的死亡率。然后，为了研究姜黄素在这种急性炎症性肝损伤模型中的潜在机制，我们仅用痤疮丙酸杆菌引发 C57BL/6 小鼠。我们发现姜黄素治疗可以减轻痤疮丙酸杆菌引起的肝损伤，这一点可以通过 ALT 产生的减少来证明。此外，姜黄素治疗减少了促炎细胞因子（例如 TNF-α 和 IFN-γ）的产生，同时减少了肝细胞凋亡。此外，姜黄素处理通过下调赖氨酸的乙酰化显着减少 HMGB1 细胞质易位和表达。综上所述，我们的结果表明姜黄素通过减少 HMGB1 细胞质易位和表达来保护小鼠免受痤疮丙酸杆菌诱导的肝损伤。 (C) 2014 Elsevier B.V. 保留所有权利。
Curcumin is a phenolic product isolated from the rhizome of Curcuma longa and has protective effects on inflammatory diseases. Here we investigated the protective effect of curcumin in acute Propionibacterium acnes (P. acnes)-induced inflammatory liver injury. C57BL/6 mice were primed with P. acnes followed by LPS challenge to induce fulminant hepatitis. Curcumin or vehicle control was administered perorally by gavage once daily starting 2 days before P. acnes priming. We found that curcumin significantly improved mouse mortality. Then, to investigate the underlying mechanisms of curcumin in this acute inflammatory liver injury model, we primed C57BL/6 mice with P. acnes only. We found that curcumin treatment attenuated P. acnes-induced liver injury as evidenced by decreased production of ALT. In addition, curcumin treatment reduced the production of proinflammatory cytokines such as TNF-alpha and IFN-gamma, accompanied by reduced hepatocyte apoptosis. Furthermore, curcumin treatment significantly reduced HMGB1 cytoplasmic translocation and expression by down-regulating acetylation of lysine. Taken together, our results suggest that curcumin protects mice from P. acnes-induced liver injury through reduction of HMGB1 cytoplasmic translocation and expression. (C) 2014 Elsevier B.V. All rights reserved.