Diversity within reference strains of Corynebacterium matruchotii includes Corynebacterium durum and a novel organism
Diversity within reference strains of Corynebacterium matruchotii includes Corynebacterium durum and a novel organism
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DOI：
10.1128/jcm.39.3.943-948.2001
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发表时间：
2001-03-01
影响因子：
9.4
通讯作者：
Coyle, MB
中科院分区：
文献类型：
--
作者：
Barrett, SLR;Cookson, BT;Coyle, MB
matruchoti棒状杆菌一直是许多牙齿发病机制研究的主题。本研究的目的是通过分析从美国类型培养收藏（ATCC）中获得的7个菌株，解决对参比菌株多样性的担忧。利用微生物ID公司的文库生成软件对全细胞脂肪酸谱进行分析，发现ATCC中沉积了三种类型的生物，分别为C、matruchotii。这三组生物也可以通过DNA-DNA斑点杂交、16S rRNA基因的两个高变区序列和吡咯烷酰基芳基酰胺酶检测来区分。这些研究表明，两株matruchotic的参考菌株ATCC 33449和ATCC 33822是新发现的硬棒状杆菌的成员。C， durum菌株的群体形态和生化反应比原来报道的更加多样化。菌株ATCC 43833是一种独特的新物种。除了C种的真正成员ATCC 14266型菌株外，matruchotii还包括ATCC菌株14265,33806和43832以及两个参考菌株L2和Richardson 13，它们构成了该物种牙齿发病机制研究中使用的绝大多数菌株。
Corynebacterium matruchotii has been the subject of numerous dental pathogenesis studies. The purpose of the present study was to resolve concerns about diversity within the reference strains of C. matruchotii through analysis of seven strains procured from the American Type Culture Collection (ATCC). Analysis of whole-cell fatty acid profiles with the library generation software of Microbial ID Inc. revealed that three types of organisms have been deposited in the ATCC as C, matruchotii. These three groups of organisms were also distinguishable by DNA-DNA dot blot hybridization, by sequences of two hypervariable regions of the 16S rRNA gene, and by the pyrrolidonyl arylamidase test. These studies indicate that two C. matruchotii reference strains, ATCC 33449 and ATCC 33822, are members of the recently proposed species, Corynebacterium durum. The colonial morphology and biochemical reactions of the C, durum strains are more diverse than originally reported. Strain ATCC 43833 is unique and represents a novel species. In addition to the type strain, ATCC 14266, true members of the species C, matruchotii include ATCC strains 14265, 33806, and 43832 plus two reference strains, L2 and Richardson 13, which comprise the vast majority of strains used in dental pathogenesis research with this species.