The persistence of electrostatically seeded endothelial cells lining a small diameter expanded polytetrafluoroethylene vascular graft
The persistence of electrostatically seeded endothelial cells lining a small diameter expanded polytetrafluoroethylene vascular graft
复制标题
DOI：
10.1106/ncqt-jfv9-2eq1-ebgu
复制
发表时间：
2001-10-01
影响因子：
2.9
通讯作者：
Rittgers, SE
中科院分区：
文献类型：
--
作者：
Bowlin, GL;Meyer, A;Rittgers, SE
Purpose: The purpose of this study was to evaluate the persistence of electrostatically seeded endothelial cells (ECs) lining an expanded polytetrafluorethylene (e-PTFE) graft after one week exposure to in vivo circulation in a canine femoral artery bypass model. This was accomplished by visualizing the PKH 26 (red fluorescent) label placed in the EC membranes prior to the seeding procedure. Furthermore, this study was performed to confirm that the source of the ECs lining the graft were those from the initial inoculum.Methods: This evaluation consisted of harvesting autologous, canine jugular vein ECs, PKH 26 labeling of the ECs, electrostatic EC seeding the e-PTFE grafts (4 mm GORE-TEX(R), Length = 6 cm), implanting the grafts (femoral artery model) for one week, and explanting the grafts fur light, fluorescent and scanning electron microscopy evaluations of the luminal surface.Results: The unseeded grafts (controls) had a mean fluorescence surface coverage of 6.82 +/-7.19%, while the EC seeded grafts had a mean of 90.3 +/- 14.3% which is significantly (p
