An expression analysis profile for the entire sucrose synthase gene family in rice
An expression analysis profile for the entire sucrose synthase gene family in rice
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DOI：
10.1016/j.plantsci.2008.02.009
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发表时间：
2008-05-01
期刊：
影响因子：
5.2
通讯作者：
Terao, Tomio
中科院分区：
文献类型：
--
作者：
Hirose, Tatsuro;Scofield, Graham N.;Terao, Tomio
此前，已对水稻中编码蔗糖合酶（Sus）（EC 2.4.1.13）的三个基因进行了鉴定，并且还报道了另外三个假定的Sus基因序列。目前的研究证实这六个基因构成了整个水稻Sus基因家族。利用实时定量RT - PCR在水稻的各种组织中进行了表达分析。在多种组织以及不同发育阶段都检测到了Sus转录本，这表明Sus参与了水稻植株众多不同的生长过程。SUS1主要在伸长组织中表达，包括根、发育中的叶片和节间；文中还讨论了SUS1在纤维素合成中的潜在作用。研究表明SUS2在多种组织中表达，这意味着它可能具有多种维持细胞基本功能的作用。此外，在缺氧（水淹条件）下萌发和生长的幼苗中，总体Sus活性以及SUS2的表达显著增加，这表明它在应对环境胁迫时对增加蔗糖代谢有一定作用。SUS3和SUS4主要在颖果中表达，这表明它们在灌浆籽粒内的碳分配中具有潜在作用。在所有检测的组织中，SUS5和SUS6的转录水平都较低，并且在水淹条件下的萌发芽中受到抑制。（C）2008爱思唯尔爱尔兰有限公司。保留所有权利。
In rice three genes encoding for sucrose synthase (Sus) (EC 2.4.1.13) have previously been characterized, and a further three sequences for putative Sus genes reported. The current work confirms that these six genes comprise the entire rice Sus gene family. An expression analysis was conducted in various rice tissues using real-time quantitative RT-PCR. Sus transcripts were detected in a wide range of tissues and at different developmental stages, indicating that Sus is involved in numerous and diverse growth processes in the rice plant. SUS1 was predominantly expressed in elongating tissues including roots, developing leaves and internodes; a potential role for SUS1 in cellulose synthesis is discussed. SUS2 was shown to be expressed in a wide range of tissues, suggesting that it may perform a number of housekeeping role(s). In addition, overall Sus activity and expression of SUS2 increased significantly in seedlings germinated and grown in hypoxia (submerged conditions), suggesting a role in increasing sucrose metabolism as a response to environmental stress. SUS3 and SUS4 were predominantly expressed in the caryopsis, indicating potential roles in carbon allocation within the filling grain. Transcript levels of SUS5 and SUS6 were low in all tissues examined, and were suppressed in germinating shoots under submergence. (C) 2008 Elsevier Ireland Ltd. All rights reserved.