Hemoglobin Modified Carbon Paste Electrode: Direct Electrochemistry and Electrocatalysis
Hemoglobin Modified Carbon Paste Electrode: Direct Electrochemistry and Electrocatalysis
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DOI：
10.1080/00032710802421640
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发表时间：
2008-11
影响因子：
2
通讯作者：
Wei Sun;Ziqin Zhai;K. Jiao
中科院分区：
文献类型：
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作者：
Wei Sun;Ziqin Zhai;K. Jiao
摘要将血红蛋白（Hb）与碳粉和石蜡均匀混合，制备了一种新型血红蛋白（Hb）修饰碳糊（CP）电极。为了防止Hb从电极表面泄漏，进一步在Hb-碳复合膏状电极表面涂敷了一层Nafion膜。在pH 7.0的磷酸盐缓冲溶液（PBS）中，血红素Fe(III)/Fe（II）偶出现了一对明确的准可逆氧化还原峰，形式电位（E0′）为−0.335 V （vs.饱和甘汞电极；CE）。制备的Hb修饰电极对三氯乙酸（TCA）和H2O2的还原表现出良好的电催化能力。
Abstract A new hemoglobin (Hb) modified carbon paste (CP) electrode was fabricated by simply mixing the hemoglobin with carbon powder and paraffin homogeneously. To prevent the leakage of Hb from the electrode surface, a Nafion film was further applied on the surface of Hb-carbon composite paste electrode. Direct electrochemistry of hemoglobin in the paste electrode was easily achieved, and a pair of well-defined quasi-reversible redox peak of heme Fe(III)/Fe(II) couple appeared with the formal potential (E0′) as −0.335 V (vs. Saturated calomel electrode; CE) in pH 7.0 phosphate buffer solution (PBS). The fabricated Hb modified electrode showed good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) and H2O2.