Testosterone effects on renal norepinephrine content and release in rats with different Y chromosomes.
Testosterone effects on renal norepinephrine content and release in rats with different Y chromosomes.
复制标题
睾酮对不同 Y 染色体大鼠肾脏去甲肾上腺素含量和释放的影响。
DOI：
10.1161/01.hyp.32.5.880
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Ely,D
中科院分区：
文献类型：
--
作者：
Jones,TJ;Dunphy,G;Milsted,A;Ely,D
- 自发性高血压大鼠（SHR）和易卒中大鼠的Y染色体已被证明含有有助于高血压效应的位点;交感神经系统和睾酮都可能参与其中。本研究的目的是观察睾酮对不同Y染色体背景的离体灌流肾去甲肾上腺素（NE）释放和含量的影响。研究涉及雄性SHR、Wistar-Kyoto大鼠（WKY）和具有不同Y染色体的2个同源品系（每组n=5至8）。将成年动物阉割，并将含有丙酸睾酮的植入物放置在颈部基部。去势后2周用放射免疫法测定血睾酮水平。分离左肾，并在恒定流量和温度下用含氧Krebs溶液灌注，同时用KCl和肾神经电刺激。收集灌注液并通过高效液相色谱法分析NE。进行乳酸脱氢酶分析，作为潜在组织损伤的标志物。肾灌流液和肾组织NE水平显着升高睾酮。单睾酮植入物的平均NE增加为13.2 ng/mL，双睾酮植入物的平均NE增加为29.8 ng/mL。与WKY Y染色体相比，SHR的Y染色体使肾脏NE释放显著增加。所有组之间均显示出显著性：1个植入物对2个植入物，P=0.0067; 1个植入物对假植入物，P=0.015; 2个植入物对假植入物，P<0.001。总之，睾酮引起增强的肾脏NE释放是应变特异性的，与Y染色体提高肾脏NE含量和释放。
—The Y chromosome in spontaneously hypertensive rats (SHR) and stroke-prone rats has been shown to contain a locus that contributes to the hypertensive effect; both the sympathetic nervous system and testosterone may be involved. The objective of this study was to look at the effects of testosterone on renal norepinephrine (NE) release and content in the isolated perfused kidney in different Y chromosome backgrounds. The study involved male SHR, Wistar-Kyoto rats (WKY), and 2 consomic strains with different Y chromosomes (n=5 to 8 per group). Adult animals were castrated, and implants containing testosterone propionate were placed at the base of the neck. Blood testosterone levels were measured by radioimmunoassay 2 weeks after castration. The left kidney was isolated and perfused with oxygenated Krebs solution at a constant flow and temperature with KCl and electrical stimulation of the renal nerves. Perfusate was collected and analyzed for NE by high-performance liquid chromatography. Lactate dehydrogenase analyses were performed as a marker for potential tissue damage. Renal perfusate and renal tissue NE levels were significantly elevated by testosterone. The average NE increase with a single testosterone implant was 13.2 ng/mL, and for a double testosterone implant it was 29.8 ng/mL. The Y chromosome from the SHR produced a significant increase in renal NE release compared with the WKY Y chromosome. Significance was shown between all groups: 1 versus 2 implants,P=0.0067; 1 versus sham implants,P=0.015; 2 versus sham implants,P<0.001. In conclusion, testosterone caused an enhanced renal NE release that was strain-specific, with the Y chromosome raising renal NE content and release.