Metabolomics analysis based on UHPLC-Q-TOF-MS/MS reveals effects of genistein on reducing mycotoxin citrinin production by Monascus aurantiacus Li AS3.4384
Metabolomics analysis based on UHPLC-Q-TOF-MS/MS reveals effects of genistein on reducing mycotoxin citrinin production by Monascus aurantiacus Li AS3.4384
复制标题
基于 UHPLC-Q-TOF-MS/MS 的代谢组学分析揭示金雀异黄素对减少金红曲霉 Li AS3.4384 霉菌毒素桔霉素产生的影响
DOI：
10.1016/j.lwt.2020.109613
复制
发表时间：
2020-08-01
影响因子：
6
通讯作者：
Huang, Zhibing
中科院分区：
文献类型：
--
作者：
He, Shanshan;Liu, Xin;Huang, Zhibing
红曲霉菌产生有用的生物活性化合物，如红曲色素、莫纳可林K和麦角固醇。采用超高效液相色谱-飞行时间质谱联用技术（UHPLC-Q-TOF-MS/MS），通过代谢组学分析，研究了染料木素在不同发酵时间诱导的红曲菌（Monacolaurantiacus Li AS 3. 4384，MALA）代谢产物的变化。共有21种显著的差异代谢物参与了最重要的代谢途径（影响>0.1）。这些代谢产物影响天冬氨酸、谷氨酸途径、三羧酸（TCA）循环、丙酮酸合成途径和赖氨酸降解途径。因此，染料木素的加入可能改变了Monoclonal桔霉素合成的前体。这些研究结果提供了显着的深入了解受染料木素影响的Monoclonal代谢途径，并将有助于提高食品和补充剂中Monoclonal的质量控制和安全性。
Monascus mold produces useful bioactive compounds such as Monascus pigments, monacolin K, and ergosterol. In this study, metabolomics analysis was used to investigate the metabolic profile changes in Monascus aurantiacus Li AS3.4384 (MALA) induced by genistein at different fermentation times using UHPLC-Q-TOF-MS/MS. The addition of genistein was found to not only reduce citrinin but also suppress significant differential metabolites. A total of 21 significant differential metabolites were involved in the most significant metabolic pathways (impact >0.1). These metabolites affected the aspartic acid, glutamic acid pathway, tricarboxylic acid (TCA) cycle, pyruvate synthesis pathway, and lysine degradation pathway. Thus, the addition of genistein may change the precursor for citrinin synthesis of Monascus. These findings provide significant insight into the Monascus metabolic pathways affected by genistein and will help to improve the quality control and safety of Monascus in food and supplements.