EFFECT OF PLASMALEMMA ATPASE INHIBITORS, DIETHYLSTILBESTROL AND ORTHO-VANADATE, ON FUSICOCCIN-INDUCED H+ EXTRUSION IN MAIZE ROOTS
EFFECT OF PLASMALEMMA ATPASE INHIBITORS, DIETHYLSTILBESTROL AND ORTHO-VANADATE, ON FUSICOCCIN-INDUCED H+ EXTRUSION IN MAIZE ROOTS
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DOI：
10.1016/0304-4211(81)90103-6
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
LADO, P
中科院分区：
文献类型：
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作者：
COLOMBO, R;BONETTI, A;LADO, P
为了探讨梭孢菌素（FC）诱导的高等植物产电H ~+排出和K ~+吸收依赖质膜ATP酶活性的可能性，研究了2种非线粒体膜ATP酶抑制剂己烯雌酚（DES）和钒酸盐对玉米根段H ~+和K ~+运输的影响。DES和钒酸盐均强烈抑制FC对H+排出和K+吸收的刺激作用。钒酸盐抑制FC诱导的H+挤出的亲脂性阳离子，铵（TBBA+）的存在下，在K+的情况下。ATP和丙酮酸水平的平行测定表明，这两种物质对转运的抑制作用似乎不依赖于对能量代谢的影响。FC刺激的H+排出和K+吸收可能依赖于质膜ATP酶的激活，催化与K+流入电耦合的产电H+单端口。
In order to investigate the possibility that fusicoccin(FC)-induced electrogenic H+ extrusion and K+ uptake in higher plants depend on the activity of a plasmalemma ATPase, the effect of 2 nonmitochondrial membrane-bound ATPase inhibitors, diethylstilbestrol (DES) and vanadate, on the transport of H+ and K+ in maize root segments was studied. Both DES and vanadate strongly inhibited the stimulating effect of FC on H+ extrusion and K+ uptake. Vanadate inhibited the FC-induced H+ extrusion in the presence of a lipophilic cation, ammonium (TBBA+) and in the absence of K+. The parallel determination of ATP and pyruvate levels showed that the inhibiting effect of the 2 substances on transport did not seem to depend on an effect on energy metabolism. FC-stimulated H+ extrusion and K+ uptake may depend on the activation of a plasmalemma ATPase, catalyzing an electrogenic H+ uniport, electrically coupled with K+ influx.