The Involvement of TRIB3 and FABP1 and Their Potential Functions in the Dynamic Process of Gastric Cancer.
The Involvement of TRIB3 and FABP1 and Their Potential Functions in the Dynamic Process of Gastric Cancer.
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DOI：
10.3389/fmolb.2021.790433
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发表时间：
2021
影响因子：
5
通讯作者：
Gong Y
中科院分区：
文献类型：
--
作者：
Liu S;Ni C;Li Y;Yin H;Xing C;Yuan Y;Gong Y
背景：TRIB3和FABP1的异常表达在人类癌症组织中已经被观察到。然而，关于它们在动态胃病中的表达变化及其功能作用的研究很少。方法：收集479例患者的组织，其中GS 89例，IM-GA 102例，EGC 144例，AGC 144例。免疫组化染色检测TRIB3和FABP1蛋白表达。同时，利用R软件和一些网络公共数据库（如TCGA和DAVID）进一步分析TRIB3和FABP1在GC中的潜在功能。结果：在从GS到EGC的多阶段过程中，TRIB3和FABP1蛋白的表达趋势为GS > IM-GA > EGC。此外，TRIB3蛋白在AGC中的表达持续下降，而FABP1的表达异常升高。Hp感染与TRIB3和FABP1表达降低显著相关。此外，这两项指标联合诊断EGC的诊断效率高于单一指标。生存分析显示，TRIB3或FABP1表达越高，胃癌预后越好。TRIB3与FABP1蛋白表达呈显著正相关。此外，CEACAM5和PRAP1与TRIB3和FABP1的表达均呈正相关，而GABRP和THBS4的表达均呈负相关。巨噬细胞M0浸润与TRIB3和FABP1表达均呈正相关。结论：TRIB3和FABP1蛋白表达随胃病进展逐渐降低，且呈正相关。Hp感染可降低TRIB3和FABP1蛋白的表达。结合TRIB3和FABP1表达可提高EGC的诊断效率。高表达TRIB3或FABP1表明胃癌预后较好。TRIB3和FABP1可能与CEACAM5、PRAP1、GABRP和THBS4相互作用，通过调节免疫细胞影响肿瘤免疫微环境，参与胃癌的发生进展。
Background: Dysregulated expression of TRIB3 and FABP1 have been previously observed in human cancer tissues. However, there are little information as to their expression change in dynamic gastric diseases and the functional roles. Methods: Tissues from a total of 479 patients, including 89 GS, 102 IM-GA, 144 EGC, and 144 AGC were collected. The protein expressions of TRIB3 and FABP1 were detected by immunohistochemical staining. Meanwhile, the potential functions of TRIB3 and FABP1 in GC were further analyzed by R software and some internet public databases, such as TCGA and DAVID. Results: During this multi-stage process that go through GS to EGC, the expression trend of TRIB3 and FABP1 protein was GS > IM-GA > EGC. Besides, the expression of TRIB3 protein continued to decrease in AGC, while the expression of FABP1 was abnormally increased. Hp infection was significantly associated with the decreased expression of TRIB3 and FABP1. In addition, the diagnostic efficiency of the combination of these two indicators to diagnose EGC was higher than that of a single indicator. Survival analysis showed that higher expression of TRIB3 or FABP1 could indicate a better prognosis of GC. The protein expressions of TRIB3 and FABP1 were significantly positively correlated. Moreover, CEACAM5 and PRAP1 were positively correlated with both TRIB3 and FABP1 expressions, while GABRP and THBS4 were negatively correlated. The macrophages M0 infiltration was positively correlated with both TRIB3 and FABP1 expressions. Conclusion: The protein expressions of TRIB3 and FABP1 gradually decreased with the gastric disease progress, and was positively correlated. Hp infection may reduce the protein expression of TRIB3 and FABP1. Combing TRIB3 and FABP1 expressions can improve the diagnostic efficiency for EGC. Either a high expression of TRIB3 or FABP1 indicates a better prognosis for GC. TRIB3 and FABP1 may interact with CEACAM5, PRAP1, GABRP and THBS4, and affect tumor immune microenvironment by regulating immune cells, and participate in the development and progression of GC.