Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva
Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva
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DOI：
10.1248/bpb.34.13
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发表时间：
2011-01-01
影响因子：
2
通讯作者：
Yanoshita, Ryohei
中科院分区：
文献类型：
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作者：
Ogawa, Yuko;Miura, Yuri;Yanoshita, Ryohei
唾液中含有大量的蛋白质，参与保护口腔组织。外泌体是由多种细胞类型分泌的具有内体来源的限制膜的小囊泡（直径30-100 μ π ι）。我们最近证明了外泌体存在于人的整个唾液中。在这项研究中，我们发现整个唾液含有至少两种类型的外泌体（外泌体I和外泌体II），它们在大小和蛋白质组成上不同。蛋白质组学分析表明，这两种类型的外泌体含有阿利克斯，Tsg 101和Hsp 70，所有外泌体标记物，免疫球蛋白A和多聚免疫球蛋白受体，但它们具有不同的蛋白质组成。在整个唾液中，大多数二肽基肽酶IV（称为CD 26）存在于外泌体II上，并且在切割趋化因子（CXCL 11和CXCL 12）中具有代谢活性。人全唾液exosomes可能参与生物活性肽的催化，在口腔局部免疫防御中发挥调节作用。
Saliva contains a large number of proteins that participate in the protection of oral tissue. Exosomes are small vesicles (30-100 urn in diameter) with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We have recently demonstrated that exosomes are present in human whole saliva. In this study, we found that whole saliva contained at least two types of exosomes (exosome I and exosome II) that are different in size and protein composition. Proteomic analysis revealed that both types of exosomes contained Alix, Tsg101 and Hsp70, all exosomal markers, immunoglobulin A and polymeric immunoglobulin receptor, whereas they had different protein compositions. Most of dipeptidyl peptidase IV known as CD26 in whole saliva, was present on the exosome II and metabolically active in cleaving chemokines (CXCL11 and CXCL12). Human whole saliva exosomes might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.