Susceptibility of European pear cultivars to Pseudomonas syringae pv. syringae using immature fruit and detached leaf assays
Susceptibility of European pear cultivars to Pseudomonas syringae pv. syringae using immature fruit and detached leaf assays
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DOI：
10.1023/a:1023574219069
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发表时间：
2003-05-01
影响因子：
1.8
通讯作者：
Montesinos, E
中科院分区：
文献类型：
--
作者：
Moragrega, C;Llorente, I;Montesinos, E
以33个梨品种和2个梨砧木为试材，对4个梨假单胞菌致病力菌株进行了感病性测定。以离体未成熟果实和幼叶为材料进行鉴定。这四个菌株的致病力相似，没有表现出品种特异性，但它们分离自不同的梨品种，并表现出不同的生化谱。最常种植的品种为Conference、Abate Fetel、General Leclerc、威廉姆斯、D. Comice，埃尔多拉多，Alexandrine，B. Anjou、PasseCrassane和砧木OHxF333对黄瓜疫霉菌致病变种感病。你好最大的严重度值上获得的'Preguystar'叶（约90%）。砧木冬莲不太感病。未成熟果实和离体叶片测定结果与田间观察品种对细菌性稻瘟病的易感性一致。然而，离体叶片试验给出了更准确的预测，并具有症状发展迅速（48小时），叶片比果实可持续更长时间的优点。该方法可作为梨品种对细菌性稻瘟病敏感性的快速、重复性好的筛选体系。
The susceptibility of thirty-three pear cultivars and two pear rootstocks to four virulent strains of Pseudomonas syringae pv. syringae was evaluated by inoculating detached immature fruits and young leaves. The four strains were similarly virulent and did not show cultivar specificity although they were isolated from different pear cultivars and exhibited different biochemical profiles. The most frequently planted pear cultivars, Conference, Abate Fetel, General Leclerc, Williams, D. Comice, El Dorado, Alexandrine, B. Anjou, Passe Crassane and the rootstock OHxF 333 were susceptible to P. syringae pv. syringae. Maximal severity values were obtained on 'Preguystar' leaves ( about 90%). The rootstockWinter Nelis was less susceptible. Results with immature fruit and detached leaf assays agreed with field observations on cultivar susceptibility to bacterial blast. However, the detached leaf test gave a more accurate prediction and has the advantages that symptoms develop quickly ( 48 h), and leaves are available for a longer period of time than fruits. This method is proposed as a rapid and reproducible screening system of cultivar susceptibility to bacterial blast of pear.