Isolation and characterization of extremely halophilic CO-oxidizing Euryarchaeota from hypersaline cinders, sediments and soils and description of a novel CO oxidizer, Haloferax namakaokahaiae Mke2.3T, sp nov.
Isolation and characterization of extremely halophilic CO-oxidizing Euryarchaeota from hypersaline cinders, sediments and soils and description of a novel CO oxidizer, Haloferax namakaokahaiae Mke2.3T, sp nov.
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DOI：
10.1093/femsec/fiw028
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发表时间：
2016-04-01
影响因子：
4.2
通讯作者：
Myers, M. R.
中科院分区：
文献类型：
--
作者：
McDuff, S.;King, G. M.;Myers, M. R.
在含有3.8M氯化钠的介质中，对高盐分土壤和沉积物中负责好氧CO氧化的微生物的系统发育进行了评估。从智利的波纳维尔盐滩(UT)和阿塔卡马沙漠盐场(Atakama Desert Salterns)分离到4株共氧化细菌：Haloarcula、Halorubrum、Haloterrigena和Natronorubrum。从夏威夷盐碱渣中分离到一株嗜盐真核细菌Haloferax菌株Mke2.3(T)。Haloferax菌株Mke2.3(T)与Haloferax larsenii JCM 13917T亲缘关系最近(16S rRNA序列同源性为97.0%)。它在有限的底物范围内生长，并在顶空浓度为0.1%时氧化CO。然而，它并没有在CO作为唯一的碳和能源来源的情况下增长。其氧化CO的能力、极脂组成、底物利用等一系列特性使其有别于H.larsenii JCM 13917T，并支持将该新菌株命名为Haloferax namakaokhaiae Mke2.3(T)，sp.11月份(=帝斯曼29988，=1MG 29162)。‘Naronorubrum thiooxans’HG1(Sorokin，Tourova和Muyzer 2005)、N.bangense(Xu，周和Tian 1999)和N.Sulfidifaciens AD2T(Cui等人)的CO氧化也被记录在案。2007)。综上所述，这些结果建立了以前未曾预料到的极端嗜盐好氧CO氧化能力，并表明这一特征可能在盐杆菌科中广泛存在，并出现在广泛的高盐度生境中。
The phylogenetic affiliations of organisms responsible for aerobic CO oxidation in hypersaline soils and sediments were assessed using media containing 3.8 M NaCl. CO-oxidizing strains of the euryarchaeotes, Haloarcula, Halorubrum, Haloterrigena and Natronorubrum, were isolated from the Bonneville Salt Flats (UT) and Atacama Desert salterns (Chile). A halophilic euryarchaeote, Haloferax strain Mke2.3(T), was isolated from Hawai'i Island saline cinders. Haloferax strain Mke2.3(T) was most closely related to Haloferax larsenii JCM 13917T (97.0% 16S rRNA sequence identity). It grew with a limited range of substrates, and oxidized CO at a headspace concentration of 0.1%. However, it did not grow with CO as a sole carbon and energy source. Its ability to oxidize CO, its polar lipid composition, substrate utilization and numerous other traits distinguished it from H. larsenii JCM 13917T, and supported designation of the novel isolate as Haloferax namakaokahaiae Mke2.3(T), sp. nov (= DSM 29988, = LMG 29162). CO oxidation was also documented for `Natronorubrum thiooxidans' HG1 (Sorokin, Tourova and Muyzer 2005), N. bangense (Xu, Zhou and Tian 1999) and N. sulfidifaciens AD2T (Cui et al. 2007). Collectively, these results established a previously unsuspected capacity for extremely halophilic aerobic CO oxidation, and indicated that the trait might be widespread among the Halobacteriaceae, and occur in a wide range of hypersaline habitats.