Evaluation of Minimum Inhibitory Concentrations for 154 Mycoplasma synoviae isolates from Italy collected during 2012-2017
Evaluation of Minimum Inhibitory Concentrations for 154 Mycoplasma synoviae isolates from Italy collected during 2012-2017
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DOI：
10.1371/journal.pone.0224903
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发表时间：
2019-11-07
期刊：
影响因子：
3.7
通讯作者：
Vicenzoni, Gaddo
中科院分区：
文献类型：
--
作者：
Catania, Salvatore;Bottinelli, Marco;Vicenzoni, Gaddo
滑膜支原体(MS)是家禽中的一种高度流行的细菌，可引起疾病和严重的经济损失。抗生素治疗是可用于控制无MS鸡群临床暴发的控制策略之一，特别是因为这种细菌可以在卵子中传播。因此，对于兽医来说，了解流通菌株的抗生素敏感性变得非常重要，以便选择最合适的一线抗生素分子作为对抗抗生素耐药性的主动作用。我们评估了2012-2017年间在意大利收集的MS分离株对恩诺沙星、土霉素、强力霉素、红霉素、泰乐菌素、替米考星、螺旋霉素、替米考林、氟苯尼考和林可霉素的最低抑菌浓度(MIC)。使用商业MIC平板对来自不同家禽商业类别(肉鸡、蛋鸡和火鸡)的154株MS分离物进行了测试。所有MS分离株对红霉素(MIC90)和恩诺沙星(MIC90)均有很高的MIC值(MIC90和MIC90分别为8mU/m L和16mU/m L)。测得的多西环素和土霉素的MIC值是相互叠加的，只有稀释差一倍。泰乐菌素和替米考星的MIC值存在差异。有趣的是，有7个菌株对林可霉素和替米考星有很高的MIC值，但并非所有菌株都有很高的泰乐菌素MIC值。大多数MS菌株对螺旋霉素的MIC值较低，但有7株菌株的MIC和Gt；=16mU g/m L。在观察期内，不同MIC类别的出现频率因所测试的抗生素而异。有趣的是，替米考星的最低抑菌浓度(MICs)清楚地显示出一种随时间变化的逐步向高浓度类转移的趋势，这表明MS分离株之间正在进行选择过程。在标准化的断点出现以便于数据解释之前，在此期间继续研究MIC值的波动将是基本的，以便创建一个重要的数据库，该数据库将有助于兽医选择适当的药物来治疗这种有影响力的支原体。
Mycoplasma synoviae (MS) is a highly prevalent bacterial species in poultry causing disease and severe economic losses. Antibiotic treatment is one of the control strategies that can be applied to contain clinical outbreaks in MS-free flocks, especially because this bacterium can be transmitted in ovo. It becomes, then, very important for veterinarians to know the antibiotic susceptibility of the circulating strains in order to choose the most appropriate first-line antibiotic molecule as a proactive role in fighting antibiotic resistance. We evaluated the Minimum Inhibitory Concentrations (MICs) of enrofloxacin, oxytetracycline, doxycycline, erythromycin, tylosin, tilmicosin, spiramycin, tiamulin, florfenicol and lincomycin for MS isolates collected between 2012 and 2017 in Italy. A total of 154 MS isolates from different poultry commercial categories (broiler, layer, and turkey sectors) was tested using commercial MIC plates. All MS isolates showed very high MIC values of erythromycin (MIC90 >= 8 mu g/mL) and enrofloxacin (MIC90 >= 16 mu g/mL). MIC values of doxycycline and oxytetracycline obtained were superimposable to each other with only a one-fold dilution difference. Discrepancies between MIC values of tylosin and tilmicosin were observed. Interestingly, seven isolates showed very high MIC values of lincomycin and tilmicosin, but not all of them showed very high MIC values of tylosin. Most of the MS isolates showed low MIC values of spiramycin, but seven strains showed a MIC >= 16 mu g/mL. In the observation period, the frequency of the different MIC classes varied dependently on the tested antibiotic. Interestingly, tilmicosin MICs clearly showed a time-dependent progressive shift towards high-concentration classes, indicative of an on-going selection process among MS isolates. Until standardized breakpoints become available to facilitate data interpretation, it will be fundamental to continue studying MIC value fluctuations in the meantime in order to create a significant database that would facilitate veterinarians in selecting the proper drug for treating this impactful Mycoplasma.