A switchable yeast display/secretion system.
A switchable yeast display/secretion system.
复制标题
DOI：
10.1093/protein/gzv043
复制
发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
J. V. Van Deventer;Ryan L. Kelly;Saravanan Rajan;K. Wittrup;S. Sidhu
中科院分区：
文献类型：
--
作者：
J. V. Van Deventer;Ryan L. Kelly;Saravanan Rajan;K. Wittrup;S. Sidhu
Display technologies such as yeast and phage display offer powerful alternatives to traditional immunization-based antibody discovery, but require conversion of displayed proteins into soluble form prior to downstream characterization. Here we utilize amber suppression to implement a yeast-based switchable display/secretion system that enables the immediate production of soluble, antibody-like reagents at the end of screening efforts. Model selections in the switchable format remain efficient, and library screening in the switchable format yields renewable sources of affinity reagents exhibiting nanomolar binding affinities. These results confirm that this system provides a seamless link between display-based screening and the production and evaluation of soluble forms of candidate binding proteins. Switchable display/secretion libraries provide a cloning-free, accessible approach to affinity reagent generation.
