Multiple mRNAs are generated from the chicken lysozyme gene
Multiple mRNAs are generated from the chicken lysozyme gene
复制标题
鸡溶菌酶基因产生多种 mRNA
DOI：
10.1016/0092-8674(81)90182-3
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发表时间：
1981
期刊：
影响因子：
64.5
通讯作者：
A. E. Sippel
中科院分区：
文献类型：
--
作者：
M. Grez;H. Land;K. Giesecke;G. Schütz;Alexander Jung;A. E. Sippel
我们测定了鸡溶菌酶基因5 ′侧翼区一个770 bp的Pst Ⅰ片段的DNA序列，该片段含有450个核苷酸。进行核酸酶作图以定位含有溶菌酶特异性序列的核RNA和mRNA的5 '端。我们提出的证据表明，5 '非编码区的鸡溶菌酶mRNA的长度是异质的。不同mRNA的5 '末端定位在其共同起始密码子上游的29、31和53个核苷酸。溶菌酶特异性核RNA的5 '端定位在与mRNA相似的位置。AT富集区和与E. coli RNA聚合酶识别序列在这些5 '末端的上游约30和70个核苷酸处被发现。然而，富含AT的区域与典型的Goldberg-Hogness盒不同，因为它们不包含极其保守的TATA序列基序。在溶菌酶、伴清蛋白和卵清蛋白基因的5 '端的序列比较揭示了只有一个部分同源的区域，在mRNA起始位点上游140个核苷酸。溶菌酶是在类固醇激素的控制下在鸡输卵管的管状腺细胞中合成的主要卵白色蛋白之一（Palmiter，1972）。在向未成熟鸡施用雌激素后，卵清蛋白、伴清蛋白、卵类粘蛋白和溶菌酶的细胞mRNA浓度显著增加（Palmiter，1975;罗森和O 'Malley，1975; Schimke等人，1975年; Hynes等人，1977年，1979年）。编码卵白色蛋白的mRNA的积累是由于卵白色蛋白基因的转录激活以及其mRNA稳定性的改变（Palmiter，1974; Nguyen-Huu et al.，1978; Hynes等人，1979; Schlitz等人，1978; Swaneck等人，1979年）。
We have determined the DNA sequence of a 770 bp Pst I fragment containing 450 nucleotides of the 5’flanking region of the chicken lysozyme gene. Slnuclease mapping was performed to localize the 5’end of nuclear RNA containing lysozyme-specific sequences and of the mRNA. We present evidence that the 5’noncoding region of the chicken lysozyme mRNA is heterogeneous in length. The 5’termini of the different mRNAs map 29, 31 and 53 nucleotides upstream from their common initiation codon. The 5’ends of lysozyme-specific nuclear RNAs map at positions similar to that of the mRNA. AT-rich regions and sequences similar to the E. coli RNA polymerase recognition sequence are found around 30 and 70 nucleotides upstream from each of these 5’termini. The AT-rich regions differ, however, from the canonical Goldberg-Hogness box in that they do not contain the extremely conserved TATA sequence motif. Sequence comparison at the 5’end of the lysozyme, conalbumin and ovalbumin genes reveals only one region of partial homology, 140 nucleotides upstream from the mRNA start sites.Lysozyme is one of the major egg white proteins synthesized in the tubular gland cells of the chicken oviduct under the control of steroid hormones (Palmiter, 1972). Following administration of estrogens to immature chicken, the cellular mRNA concentration for ovalbumin, conalbumin, ovomucoid and lysozyme increases dramatically(Palmiter, 1975; Rosen and O’Malley, 1975; Schimke et al., 1975; Hynes et al., 1977, 1979). The accumulation of the mRNA coding for the egg white proteins is due to transcriptional activation of the egg white protein genes as well as to alterations in the stability of their mRNAs (Palmiter, 1974; Nguyen-Huu et al., 1978; Hynes et al., 1979; Schlitz et al., 1978; Swaneck et al., 1979).
母鸡卵清蛋白信使核糖核酸 5 末端的异质性。
影响因子：
14.9
作者：
Malek,LT;Eschenfeldt,WH;Munns,TW;Rhoads,RE
通讯作者：
Rhoads,RE