Investigation of affinity partition chromatography using formate dehydrogenase as a model.
Investigation of affinity partition chromatography using formate dehydrogenase as a model.
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使用甲酸脱氢酶作为模型研究亲和分配色谱。
DOI：
10.1016/0021-9673(90)85015-n
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kula,MR
中科院分区：
文献类型：
--
作者：
Walsdorf,A;Forciniti,D;Kula,MR
用亲和层析法从博伊丁假丝酵母（Candida boidinii）粗提物中纯化甲酸脱氢酶（FDH）。通过在色谱柱的移动的相中加入聚乙二醇-Procion Red HE 3b作为亲和配体，选择性地增加酶的分配系数K。K值的增加导致酶-配体复合物的早期洗脱，并将目标蛋白与污染物的主峰分离。
The enzyme formate dehydrogenase (FDH) was purified from the crude extract ofCandida boidiniiby affinity partition chromatography. The partition coefficient,K, of the enzyme was selectively increased by adding polyethylene glycol—Procion Red HE3b as an affinity ligand to the mobile phase in the chromatographic column. The increasedKvalue led to early elution of the enzyme—ligand complex and separated the target protein from the main peak of the contaminants.