Purification and in vitro anti-proliferative effect of novel neutral polysaccharides from Lentinus edodes
Purification and in vitro anti-proliferative effect of novel neutral polysaccharides from Lentinus edodes
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DOI：
10.1016/j.ijbiomac.2012.10.007
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发表时间：
2013-01-01
影响因子：
8.2
通讯作者：
Zhou, Yifa
中科院分区：
文献类型：
--
作者：
Jeff, Iteku Bekomo;Yuan, Xiaowen;Zhou, Yifa
采用热水提取、乙醇沉淀、阴离子交换层析等方法从香菇担子果中分离得到1 (1 - bbb6)- β - d -葡聚糖（WPLE-N-1）和2个甘露半乳糖葡聚糖（WPLE-N-2和WPLE-N-3），并采用凝胶渗透层析（GPC）对其进行纯化。采用高效液相色谱（HPLC）、高效凝胶渗透色谱（HPGPC）、甲基化分析、高酸盐氧化-史密斯降解、傅里叶变换-红外（FT-IR）和核磁共振（包括二维核磁共振）等方法研究了它们的结构特征。HPLC分析表明，WPLE-N-1主要由葡萄糖（92%）、少量半乳糖（3.9%）和甘露糖（4.1%）组成，WPLE-N-2和WPLE-N-3的甘露糖-半乳糖-葡萄糖的摩尔比分别为10:27:63和5:12:83。GPC和HPGPC结果表明，WPLE-N-1、WPLE-N-2和WPLE-N-3为均匀组分，分子量分别为757.5 kDa、20.9 kDa和4.7 kDa。化学和光谱研究表明，WPLE-N-1由(1 - bbb6)- β - d -葡萄糖吡喃基残基组成；而wle - n -2和wle - n -3则含有(1 - bbb6)-、(1 ->)-和(1 ->)- α - d -葡萄糖吡喃基残基、(1 - bbb6)- α - d -半乳糖吡喃基残基、(1 -> 3,6)-和(1 -> 2,4)- α - d -甘露吡喃基残基和d -葡萄糖吡喃基残基的末端残基。初步生物活性试验表明，这3种多糖对S-180肉瘤、HCT-116和HT-29具有体外抗肿瘤活性。这一发现表明甘露半乳糖葡聚糖应该作为潜在的抗肿瘤药物进行探索，并在食品和制药工业中作为肿瘤细胞生长抑制剂加以利用。(C) 2012 Elsevier B.V.版权所有
A (1 -> 6)-beta-D-glucan (WPLE-N-1) and two mannogalactoglucans (WPLE-N-2 and WPLE-N-3) were isolated from the basidiocarps of Lentinus edodes by hot water extraction, ethanol precipitation, anion exchange chromatography, and further purified by gel-permeation chromatography (GPC). Their structural features were investigated by high performance liquid chromatography (HPLC), high performance gel-permeation chromatography (HPGPC), methylation analysis, periodate oxidation-Smith degradation, Fourier transform-infrared (FT-IR) and NMR spectroscopy, including two-dimensional (2D) NMR. HPLC analysis revealed that WPLE-N-1 was mainly composed of glucose (92%) with small amount of galactose (3.9%) and mannose (4.1%), WPLE-N-2 and WPLE-N-3 contained mannose-galactose-glucose in the molar ratio of 10:27:63 and 5:12:83, respectively. GPC and HPGPC showed that WPLE-N-1, WPLE-N-2 and WPLE-N-3 are homogeneous fractions and their molecular weights were estimated to be 757.5 kDa, 20.9 kDa and 4.7 kDa, respectively. Chemical and spectroscopic studies indicated that WPLE-N-1 consisted of (1 -> 6)-beta-D-glucopyranosyl residues; while WPLE-N-2 and WPLE-N-3 were found to contain (1 -> 6)-, (1 -> 4)- and (1 -> 3)-alpha-D-glucopyranosyl residues, (1 -> 6)-alpha-D-galactopyranosyl residues, (1 -> 3,6)- and (1 -> 2,4)-alpha-D-mannopyranosyl residues and terminal residues of D-glucopyranosyl residues. On a preliminary bioactivity test, these three polysaccharides exhibited antitumor activity against Sarcoma S-180, Carcinoma HCT-116 and HT-29 in vitro. This finding suggests that mannogalactoglucan should be explored as potential antitumor agents and utilized as tumor cell growth inhibitors for food and pharmaceutical industries. (C) 2012 Elsevier B.V. All rights reserved.