Characterisation by high performance liquid chromatography of circulating growth hormone releasing factors in a human plasma.
Characterisation by high performance liquid chromatography of circulating growth hormone releasing factors in a human plasma.
复制标题
通过高效液相色谱法表征人血浆中循环生长激素释放因子。
作者：
E. Penny;R. Patience;A. Sopwith;J. Wass;G. Besser;L. Rees
三种形式的循环免疫反应性人生长激素释放因子（ir-hGRF）已被确定从患者的肢端肥大症与播散性类癌肿瘤。这是第一个已知的ir-hGRF在人血浆中的分子形式的报告。高效液相色谱法（HPLC）在C3，大孔反相柱和凝胶过滤色谱法与灵敏的放射免疫测定法（RIA）结合使用。该患者血浆中ir-hGRF的浓度大大升高，为25，000 ng/l（正常范围小于60 ng/l）。血浆的凝胶过滤（G50）色谱显示与合成hGRF-40共洗脱的单峰。然而，Vycor提取的血浆的反相HPLC将ir-hGRF分解成三种组分，其与合成的hGRF-40（69%）、hGRF-44（22%）和hGRF-37（9%）共洗脱。目前尚不清楚这三种形式是否是天然变体，或者hGRF-40和hGRF-37之一或两者是否是hGRF-44的切割产物。
Three forms of circulating immunoreactive human growth hormone-releasing factor (ir-hGRF) have been identified from a patient whose acromegaly was associated with a disseminated carcinoid tumour. This is the first known report of the molecular forms of ir-hGRF in human plasma. High performance liquid chromatography (HPLC) on a C3, wide pore reversed-phase column and gel filtration chromatography were used in conjunction with a sensitive radioimmunoassay (RIA). The greatly elevated concentration of the ir-hGRF in plasma from this patient was 25,000 ng/l (normal range less than 60 ng/l). Gel filtration (G50) chromatography of the plasma revealed a single peak which coeluted with synthetic hGRF-40. However, reversed-phase HPLC of Vycor-extracted plasma resolved the ir-hGRF into three components, which coeluted with synthetic hGRF-40 (69%), hGRF-44 (22%) and hGRF-37 (9%). At present it is not clear if the three forms are natural variants or whether either or both hGRF-40 and hGRF-37 are cleavage products of hGRF-44.