RNA-seq Profiling Reveals Novel Target Genes of LexA in the Cyanobacterium Synechocystis sp. PCC 6803.
RNA-seq Profiling Reveals Novel Target Genes of LexA in the Cyanobacterium Synechocystis sp. PCC 6803.
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DOI：
10.3389/fmicb.2016.00193
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发表时间：
2016
影响因子：
5.2
通讯作者：
Hihara Y
中科院分区：
文献类型：
--
作者：
Kizawa A;Kawahara A;Takimura Y;Nishiyama Y;Hihara Y
莱克萨是大肠杆菌和其他细菌物种中由DNA损伤诱导的SOS基因的公认转录阻遏物。然而，在蓝细菌集胞藻属PCC 6803中的莱克萨已被认为不参与SOS响应。在这项研究中，我们进行了RNA-seq分析的野生型菌株和lexA破坏的突变体，以获得在集胞藻LexA调控基因的全面看法。干扰莱克萨的积极或消极影响的基因表达的各种细胞功能，如趋光运动，积累的主要相容性溶质葡糖基甘油和亚基的双向氢化酶，光系统I，和藻胆体复合物。我们还观察到，在培养后期的突变体中的铁和锰吸收相关基因的表达水平增加。然而，没有一个与DNA代谢相关的基因受到莱克萨破坏的影响。使用重组莱克萨蛋白的DNA凝胶迁移率变动分析表明，莱克萨与pilA 7、pilA 9、ggpS和slr 1670的上游区域结合以直接调节它们的表达，但是通过破坏莱克萨而引起的光系统I基因表达水平的变化可能是次级效应。
LexA is a well-established transcriptional repressor of SOS genes induced by DNA damage in Escherichia coli and other bacterial species. However, LexA in the cyanobacterium Synechocystis sp. PCC 6803 has been suggested not to be involved in SOS response. In this study, we performed RNA-seq analysis of the wild-type strain and the lexA-disrupted mutant to obtain the comprehensive view of LexA-regulated genes in Synechocystis. Disruption of lexA positively or negatively affected expression of genes related to various cellular functions such as phototactic motility, accumulation of the major compatible solute glucosylglycerol and subunits of bidirectional hydrogenase, photosystem I, and phycobilisome complexes. We also observed increase in the expression level of genes related to iron and manganese uptake in the mutant at the later stage of cultivation. However, none of the genes related to DNA metabolism were affected by disruption of lexA. DNA gel mobility shift assay using the recombinant LexA protein suggested that LexA binds to the upstream region of pilA7, pilA9, ggpS, and slr1670 to directly regulate their expression, but changes in the expression level of photosystem I genes by disruption of lexA is likely a secondary effect.