Analysis of the peripheral T-cell repertoire in kidney transplant patients
Analysis of the peripheral T-cell repertoire in kidney transplant patients
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DOI：
10.1002/eji.201040301
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发表时间：
2010-11-01
影响因子：
5.4
通讯作者：
Brouard, Sophie
中科院分区：
文献类型：
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作者：
Miqueu, Patrick;Degauque, Nicolas;Brouard, Sophie
肾移植的长期稳定性取决于无慢性排斥反应。由于T细胞在排斥过程中起关键作用，分析T细胞库可能有助于理解移植物功能的结果。因此，我们研究了一种新的统计工具的作用，该工具用于分析外周血TCR库，以确定229名接受免疫抑制的稳定肾移植患者的免疫差异。尽管根据严格的标准选择患者，但患者表现出异质性的t细胞库使用，范围从无偏倚到高度选择的TCR库；倾斜的TCR库与CD8(+)/CD4(+) t细胞比例的增加相关。t细胞库模式在临床结果相反的患者中进行了比较，即稳定的无药物手术耐受受体和“可疑”形式的体液慢性排斥患者，发现从多克隆到高度选择的TCR库分别有显著差异。此外，选定的TCR曲目被发现与班夫分数等级呈正相关。综上所述，这些数据表明，TCR全库分类可能包括在肾移植后患者随访的综合评分计算中。
The long-term stability of renal grafts depends on the absence of chronic rejection. As T cells play a key role in rejection processes, analyzing the T-cell repertoire may be useful for understanding graft function outcomes. We have therefore investigated the power of a new statistical tool, used to analyze the peripheral blood TCR repertoire, for determining immunological differences in a group of 229 stable renal transplant patients undergoing immunosuppression. Despite selecting the patients according to stringent criteria, the patients displayed heterogeneous T-cell repertoire usage, ranging from unbiased to highly selected TCR repertoires; a skewed TCR repertoire correlating with an increase in the CD8(+)/CD4(+) T-cell ratio. T-cell repertoire patterns were compared in patients with clinically opposing outcomes i.e. stable drug-free operationally tolerant recipients and patients with the "suspicious" form of humoral chronic rejection and were found significantly different, from polyclonal to highly selected TCR repertoires, respectively. Moreover, a selected TCR repertoire was found to positively correlate with the Banff score grade. Collectively, these data suggest that TCR repertoire categorization might be included in the calculation of a composite score for the follow-up of patients after kidney transplantation.