gamma/delta T cell-deficient mice have impaired mucosal immunoglobulin A responses.
gamma/delta T cell-deficient mice have impaired mucosal immunoglobulin A responses.
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DOI：
10.1084/jem.183.4.1929
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发表时间：
1996-04-01
期刊：
影响因子：
--
通讯作者：
Kiyono H
中科院分区：
文献类型：
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作者：
Fujihashi K;McGhee JR;Kweon MN;Cooper MD;Tonegawa S;Takahashi I;Hiroi T;Mestecky J;Kiyono H
小鼠的粘液组织富含表达γ/δ T细胞受体的T细胞。由于这些细胞的功能仍不清楚，我们比较了粘膜免疫反应的γ/δ T细胞受体缺陷（TCR δ-/-）小鼠与对照小鼠相同的遗传背景。在TCR δ-/-小鼠中，肠免疫球蛋白（IG）A浆细胞的频率以及血清、胆汁、唾液和粪便样品中的伊加水平显著降低。当用破伤风类毒素加霍乱毒素作为佐剂的疫苗口服免疫时，TCR δ-/-小鼠产生低得多的伊加抗体水平。相反，抗原特异性IgM和IgG抗体应答与经口免疫的对照小鼠相当。对形成针对破伤风类毒素和霍乱毒素抗原的抗体的细胞的直接评估表明，与经口免疫的对照小鼠相比，TCR δ-/-小鼠的肠固有层和派尔集合淋巴结中存在显著较低数量的伊加抗体产生细胞。在不存在γ/δ T细胞的情况下，对摄入抗原的伊加应答的选择性降低表明γ/δ细胞在粘膜免疫中的专门作用。
Mucosal tissues of mice are enriched in T cells that express the gamma/delta T cell receptor. Since the function of these cells remains unclear, we have compared mucosal immune responses in gamma/delta T cell receptor-deficient (TCRdelta-/-) mice versus control mice of the same genetic background. The frequency of intestinal immunoglobulin (Ig) A plasma cells as well as IgA levels in serum, bile, saliva, and fecal samples were markedly reduced in TCRdelta-/- mice. The TCRdelta-/- mice produced much lower levels of IgA antibodies when immunized orally with a vaccine of tetanus toxoid plus cholera toxin as adjuvant. Conversely, the antigen-specific IgM and IgG antibody responses were comparable to orally immunized control mice. Direct assessment of the cells forming antibodies against the tetanus toxoid and cholera toxin antigens indicated that significantly lower numbers of IgA antibody- producing cells were present in the intestinal lamina propria and Peyer's patches of TCRdelta-/- mice compared with the orally immunized control mice. The selective reduction of IgA responses to ingested antigens in the absence of gamma/delta T cells suggests a specialized role for gamma/delta cells in mucosal immunity.