PARAFORMALDEHYDE FIXATION OF HEMATOPOIETIC-CELLS FOR QUANTITATIVE FLOW-CYTOMETRY (FACS) ANALYSIS
PARAFORMALDEHYDE FIXATION OF HEMATOPOIETIC-CELLS FOR QUANTITATIVE FLOW-CYTOMETRY (FACS) ANALYSIS
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DOI：
10.1016/0022-1759(81)90253-2
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发表时间：
1981-01-01
影响因子：
2.2
通讯作者：
WARNER, NL
中科院分区：
文献类型：
--
作者：
LANIER, LL;WARNER, NL
确定了一种保存细胞用于后续流式细胞术 (FACS) 分析的简单方法。在 1% 多聚甲醛-0.85% 盐水溶液中固定不会显着改变细胞的库尔特体积、光散射或荧光特性。该方法适用于分析的所有细胞类型，包括小鼠、人和大鼠淋巴细胞、红细胞和转化细胞系。预先用荧光素缀合抗体染色的固定细胞可以储存在 4°C 下。在 FACS 分析之前，在黑暗中 C 至少 1 周。这种方法对于那些使用体内衍生样本或使用流式细胞仪设施有限的人来说应该是有用的。
A simple method to preserve cells for subsequent flow cytometry (FACS) analysis was determined. Fixation in 1% paraformaldehyde-0.85% saline solution did not significantly alter the Coulter volume, light scatter or fluorescence properties of the cells. This method was suitable for all cell types analyzed, including mouse, human and rat lymphoid cells, erythrocytes and transformed cell lines. Fixed cells, previously stained with fluorescein conjugated antibodies, could be stored at 4.degree. C in the dark for at least 1 wk prior to FACS analysis. This method should prove useful to those who work with in vivo derived specimens or have limited access to flow cytometry facilities.