HUMAN IMMUNE RESPONSIVENESS TO LOLIUM-PERENNE POLLEN ALLERGEN LOL-P-III (RYE-III) IS ASSOCIATED WITH HLA-DR3 AND DR5
HUMAN IMMUNE RESPONSIVENESS TO LOLIUM-PERENNE POLLEN ALLERGEN LOL-P-III (RYE-III) IS ASSOCIATED WITH HLA-DR3 AND DR5
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DOI：
10.1016/0198-8859(89)90070-0
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发表时间：
1989-05-01
期刊：
影响因子：
2.7
通讯作者：
MARSH, DG
中科院分区：
文献类型：
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作者：
ANSARI, AA;FREIDHOFF, LR;MARSH, DG
Lolium perenne（多年生黑麦草）花粉的一种经过充分表征的过敏原 Lol p III 已被用作模型抗原来研究人类免疫反应的遗传控制。在两组皮试阳性的白人成年人（N = 135 和 67）中研究了 HLA 类型与 IgE 或 IgG 抗体 (Ab) 对 Lol p III 反应性之间的关联。我们通过非参数和参数分析发现，对 Lol p III 的免疫反应与 HLA-DR3 和 DR5 显着相关。没有发现任何 DQ 类型与对 Lol p III 的免疫反应之间存在关联。 B8+、DR3+ 受试者和 B8-、DR3+ 受试者之间 Lol p III 的几何平均 IgE 或 IgG Ab 水平没有差异，表明 HLA-B8 对关联没有影响。在草抗原免疫治疗后获得的受试者的Lol p III IgG Ab数据显示，100%的DR3受试者和100%的DR5受试者为Ab+。对 DRB 基因产物的所有可用蛋白质序列的比较表明，DR3 和 DR5（以及 DRw6）的第一个高变区（以及其他区域）包含序列 Glu9-Tyr-Ser-Thr-Ser13。我们的观察结果与以下可能性一致：对变应原Lol p III的免疫应答与DR.beta.1多肽链的第一高变区中的该氨基酸序列相关。
A well-characterized allergen of Lolium perenne (perennial rye grass) pollen, Lol p III, has been used as a model antigen to study the genetic control of the human immune response. Associations between HLA type and IgE or IgG antibody (Ab) responsiveness to Lol p III were studied in two groups of skin-test-positive Caucasoid adults (N = 135 and 67). We found by nonparametric and parametric analyses that immune responsiveness to Lol p III was significantly associated with HLA-DR3 and DR5. No association was found between any DQ type and immune responsiveness to Lol p III. Geometric mean IgE or IgG Ab levels to Lol p III were not different between B8+, DR3+ subjects and B8-, DR3+ subjects, showing that HLA-B8 had no influence on the association. Lol p III IgG Ab data obtained on subjects after grass antigen immunotherapy showed that 100% of DR3 subjects and 100% of DR5 subjects were Ab+. A comparison of all the available protein sequences of DRB gene products showed that the first hypervariable region of DR3 and DR5 (and DRw6), and no other region, contains the sequence Glu9-Tyr-Ser-Thr-Ser13. Our observations are consistent with the possibility that immune responsiveness to the allergen Lol p III is associated with this amino acid sequence in the first hypervariable region of the DR.beta.1 polypeptide chain.