Active pixel sensor array for high spatio-temporal resolution electrophysiological recordings from single cell to large scale neuronal networks
Active pixel sensor array for high spatio-temporal resolution electrophysiological recordings from single cell to large scale neuronal networks
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DOI：
10.1039/b907394a
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Martinoia, Sergio
中科院分区：
文献类型：
--
作者：
Berdondini, Luca;Imfeld, Kilian;Martinoia, Sergio
本文介绍了一个基于芯片的电生理平台，该平台能够研究体外神经元准备中的微观和宏观电路。该方法基于×微电极阵列设备，提供高空间(电极间隔21 mm)和时间分辨率(从4096个微电极的0.13ms到微电极的8ms)的细胞外电生理活动记录。应用于体外神经元制备，我们展示了这种方法如何能够获得神经元信号，以研究从单个细胞和微电路到大规模神经元网络的神经元活动。该平台的主要部件是采用类似于光成像器的互补金属氧化物半导体(CMOS)技术实现的金属微电极阵列(MEA)、像素内集成的低噪声放大器(11µV-RMS)和高速随机寻址逻辑。该芯片与实时采集系统相结合，能够以7.8 kHz/电极的速度记录整个阵列并对采集的信号进行处理。
This paper presents a chip-based electrophysiological platform enabling the study of micro- and macro-circuitry in in-vitro neuronal preparations. The approach is based on a 64 x 64 microelectrode array device providing extracellular electrophysiological activity recordings with high spatial (21 mm of electrode separation) and temporal resolution (from 0.13 ms for 4096 microelectrodes down to 8 ms for 64 microelectrodes). Applied to in-vitro neuronal preparations, we show how this approach enables neuronal signals to be acquired for investigating neuronal activity from single cells and microcircuits to large scale neuronal networks. The main elements of the platform are the metallic microelectrode array ( MEA) implemented in Complementary Metal Oxide Semiconductor (CMOS) technology similar to a light imager, the in-pixel integrated low-noise amplifiers (11 mu V-rms) and the high-speed random addressing logic. The chip is combined with a real-time acquisition system providing the capability to record at 7.8 kHz/electrode the whole array and to process the acquired signals.